Cleaved Caspase-3 (Asp175) Recombinant Rabbit mAb (S-1406-409)

Caspase-3,CASP-3,Apopain,Cysteine protease CPP32 (CPP-32),Protein Yama,SREBP cleavage activity 1 (SCA-1),CPP32,CASP3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6373

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Cleaved Caspase-3 (Asp175)
分子别名

Caspase-3; CASP-3; Apopain; Cysteine protease CPP32 (CPP-32); Protein Yama; SREBP cleavage activity 1 (SCA-1); CPP32; CASP3
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P42574
克隆号

S-1406-409
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
RRID

AB_3750939
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:250 Hu, Rt
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:250	Hu, Rt
ICC	1:500	Hu

背景介绍

Cleaved Caspase-3 (Asp175) is the ~17/19 kDa proteolytically activated form of human caspase-3 that, once cleaved after Asp175-Ser176 during apoptosis, loses its N-terminal pro-domain, assembles into active hetero-tetramers (p17/p12) and relentlessly hydrolyzes Asp-x bonds in >100 cytoplasmic, nuclear and cytoskeletal substrates—such as PARP, ICAD, ROCK1 and gelsolin—thereby dismantling cellular architecture, inactivating DNA repair, triggering chromatin condensation, membrane blebbing and phosphatidyl-serine exposure, and thus serving as the pivotal executioner caspase whose appearance, detected by the widely used “Asp175” neo-epitope antibody, is universally recognized as an irreversible biochemical signature of cells committed to programmed cell death.

免疫印迹
- WB result of Cleaved Caspase-3 (Asp175) Recombinant Rabbit mAb
  Primary antibody: Cleaved Caspase-3 (Asp175) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 1 µM staurosporine for 4 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 32 kDa
  Observed MW: 17, 19 kDa
- WB result of Cleaved Caspase-3 (Asp175) Recombinant Rabbit mAb
  Primary antibody: Cleaved Caspase-3 (Asp175) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 starve overnight, then treated with 1 µM staurosporine for 3 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 32 kDa
  Observed MW: 19 kDa
- WB result of Cleaved Caspase-3 (Asp175) Recombinant Rabbit mAb
  Primary antibody: Cleaved Caspase-3 (Asp175) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated C6 whole cell lysate 20 µg
  Lane 2: C6 starve overnight, then treated with 1 µM staurosporine for 3 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 32 kDa
  Observed MW: 17 kDa
  This blot was developed with high sensitivity substrate
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-Cleaved Caspase-3 (Asp175) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-Cleaved Caspase-3 (Asp175) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Cleaved Caspase-3 (Asp175) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Cleaved Caspase-3 (Asp175) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat colon. Anti-Cleaved Caspase-3 (Asp175) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC analysis of HeLa whole cell lysate treated with 1uM staurosporine for 4 hours (top panel) and untreated HeLa cells (below panel). Anti-Cleaved Caspase-3 (Asp175) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of Cleaved Caspase-3 (Asp175) in tumor tissue.
- Expression of Cleaved Caspase-3 (Asp175) in human tissue.
- Expression of Cleaved Caspase-3 (Asp175) in mouse & rat tissue.