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Rabbit抗原名称
CD42a分子别名
Platelet glycoprotein IX; GP-IX; GPIX; Glycoprotein 9; GP9免疫原
Recombinant Protein细胞定位
MembraneAccession
P14770克隆号
S-2705-46抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
Human spleen, mouse plasma, mouse spleen纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu, Ms IHC-P 1:500 Hu, Ms IF 1:500 Hu
CD42a, also known as glycoprotein IX (GPIX), is a small, single-pass transmembrane glycoprotein expressed on platelets and megakaryocytes that non-covalently associates with glycoproteins Ibα, Ibβ, and V to form the GPIb-V-IX complex, which acts as the platelet receptor for von Willebrand factor (vWF), enabling vWF-dependent platelet adhesion to injured vessel walls and initiating arterial hemostasis; CD42a contains leucine-rich repeats, is encoded by GP9 on chromosome 3q21.3, and mutations in this gene cause Bernard–Soulier syndrome, a bleeding disorder characterized by giant platelets and impaired aggregation.
免疫印迹
WB result of CD42a Recombinant Rabbit mAb
Primary antibody: CD42a Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse plasma lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 17-22 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD42a antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-CD42a antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD42a antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-CD42a antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human spleen. Anti-CD42a antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of CD42a in tumor tissue.
Expression of CD42a in human tissue.
Expression of CD42a in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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