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Rabbit抗原名称
AGTR1分子别名
Type-1 angiotensin II receptor; AT1AR; AT1BR; Angiotensin II type-1 receptor (AT1 receptor); AGTR1A; AGTR1B; AT2R1; AT2R1B免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P30556克隆号
S-2447-22抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HEK-293, HepG2, HeLa, HT-1080, C2C12, PC-12, COS-7预测反应种属
(反应种属缩写表)Bv, Cz, Sh, GP, Dg, Rb纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:50-1:200 Hu, Ms, Rt ICC 1:500 Hu
AGTR1, the angiotensin II type 1 receptor, is a seven-transmembrane G-protein-coupled receptor that serves as the principal transducer of angiotensin II signaling in the renin–angiotensin–aldosterone system, mediating potent vasoconstriction, aldosterone and vasopressin release, renal sodium and water reabsorption, vascular smooth-muscle and cardiac hypertrophy, oxidative stress generation and sympathetic activation, thereby critically regulating systemic blood pressure and cardiovascular homeostasis; the receptor is widely expressed in kidney, heart, vasculature, adrenal gland and lung, can be activated not only by ligand binding but also by mechanical stretch, is transcriptionally up-regulated by inflammatory cytokines, undergoes endocytosis and heterodimerization that fine-tune its signaling output, and its chronic overactivity drives hypertension, cardiac and renal fibrosis, atherosclerosis and diabetic complications, while genetic polymorphisms that increase its expression or alter its regulation are linked to salt-sensitive hypertension and heightened cardiovascular risk .
免疫印迹
WB result of AGTR1 Recombinant Rabbit mAb
Primary antibody: AGTR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HT-1080 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 45 kDa
This blot was developed with high sensitivity substrateWB result of AGTR1 Recombinant Rabbit mAb
Primary antibody: AGTR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 45 kDa
This blot was developed with high sensitivity substrateWB result of AGTR1 Recombinant Rabbit mAb
Primary antibody: AGTR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 45 kDa
This blot was developed with high sensitivity substrateWB result of AGTR1 Recombinant Rabbit mAb
Primary antibody: AGTR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 45 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-AGTR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-AGTR1 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-AGTR1 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-AGTR1 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-AGTR1 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HEK-293 cells. Anti-AGTR1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of AGTR1 in tumor tissue.
Expression of AGTR1 in human tissue.
Expression of AGTR1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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