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Rabbit抗原名称
Heme Oxygenase 1分子别名
Heme oxygenase 1; HO-1Cleaved into 1 chainsHeme oxygenase 1 soluble form; HO; HO1; HMOX1免疫原
Recombinant Protein细胞定位
Endoplasmic reticulumAccession
P09601克隆号
S-2614-1抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
A549, HepG2, HT-1080, HeLa, NIH/3T3纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms
Heme Oxygenase-1 (HO-1, HSP32) is a 32 kDa stress-responsive enzyme anchored to the smooth endoplasmic reticulum that cleaves heme at the α-meso carbon to release equimolar biliverdin (rapidly reduced to bilirubin), carbon monoxide (CO) and ferrous iron, thereby not only eliminating the pro-oxidant heme but also generating potent antioxidants and the signaling gas CO; transcription of the HMOX1 gene is potently induced by myriad stressors—including heme itself, ROS, heavy metals, cytokines, hypoxia, nitric oxide, and natural electrophiles—via Nrf2/ARE, Bach1, HIF-1, AP-1 and NF-κB pathways, giving HO-1 a central cytoprotective role against oxidative and inflammatory injury in cardiovascular, neuronal, renal and hepatic tissues, modulating mitochondrial biogenesis, autophagy and immune tolerance, while its products bilirubin and CO respectively scavenge radicals and activate cGMP/PKG signaling to vasodilate, inhibit platelet aggregation and suppress apoptosis, making HO-1 a therapeutic target in sepsis, ischemia–reperfusion, transplant rejection, metabolic disorders and neurodegeneration.
免疫印迹
WB result of Heme Oxygenase 1 Recombinant Rabbit mAb
Primary antibody: Heme Oxygenase 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 50 µM arsenite for 8 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDaWB result of Heme Oxygenase 1 Recombinant Rabbit mAb
Primary antibody: Heme Oxygenase 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HT-1080 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDaWB result of Heme Oxygenase 1 Recombinant Rabbit mAb
Primary antibody: Heme Oxygenase 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 50 µM arsenite for 8 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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