Phospho-Glycogen Synthase (Ser641) Rabbit Polyclonal Antibody

Glycogen [starch] synthase,muscle,Glycogen synthase 1,GYS,GYS1

Datasheet COA

价格￥400.00 （供应商现货： 3-5个工作日）

货号 S0B6363

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-Glycogen Synthase (Ser641)
分子别名

Glycogen [starch] synthase, muscle; Glycogen synthase 1; GYS; GYS1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P13807
抗体类型

Polyclonal antibody
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, NIH/3T3, PC-12
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:500-1:1000 Hu, Ms, Rt

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:500-1:1000	Hu, Ms, Rt

背景介绍

Phospho-Glycogen Synthase (Ser641) is the inactive, phosphorylated form of glycogen synthase in which a phosphate group is covalently attached to serine-641; this single modification, catalyzed chiefly by glycogen synthase kinase-3 (GSK-3) downstream of insulin signaling, sterically hinders the enzyme’s active site and prevents the elongation of glycogen chains, thereby providing a rapid, reversible switch that links hormonal cues to systemic glucose storage.

免疫印迹
- WB result of Phospho-Glycogen Synthase (Ser641) Rabbit pAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho-Glycogen Synthase (Ser641) Rabbit pAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg (λ phosphatase treated membrane)
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 84 kDa
  Observed MW: 90 kDa
  This blot was developed with high sensitivity substrate
- WB result of Phospho-Glycogen Synthase (Ser641) Rabbit pAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho-Glycogen Synthase (Ser641) Rabbit pAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 whole cell lysate 20 µg (λ phosphatase treated membrane)
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 84 kDa
  Observed MW: 90 kDa
- WB result of Phospho-Glycogen Synthase (Ser641) Rabbit pAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho-Glycogen Synthase (Ser641) Rabbit pAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: PC-12 whole cell lysate 20 µg (λ phosphatase treated membrane)
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 84 kDa
  Observed MW: 90 kDa
  This blot was developed with high sensitivity substrate
斑点杂交
- Dot blot result of Phospho-Glycogen Synthase (Ser641) Recombinant Rabbit mAb
  Lane 1: Glycogen Synthase (Ser641) phospho peptide
  Lane 2: Glycogen Synthase (Ser641) unmodified peptide
  Primary antibody: Phospho-Glycogen Synthase (Ser641) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution