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Phospho-MEK1 (Ser298) Recombinant Rabbit mAb (S-2487-19)

Dual specificity mitogen-activated protein kinase kinase 1,MAP kinase kinase 1,MAPKK 1,MKK1,ERK activator kinase 1,MAPK/ERK kinase 1 (MEK 1),MEK1,PRKMK1,MAP2K1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6362

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-MEK1 (Ser298)
分子别名

Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1 (MEK 1); MEK1; PRKMK1; MAP2K1
免疫原

Synthetic Peptide
细胞定位

Cytoskeleton, Nucleus, Cytoplasm
Accession

Q02750
克隆号

S-2487-19
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000-1:2000 Hu, Ms, Rt
ICC 1:500 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000-1:2000	Hu, Ms, Rt
ICC	1:500	Hu

背景介绍

Phospho-MEK1 (Ser298) is the activated form of mitogen-activated protein kinase kinase-1 that carries a phosphate group on serine-298; this post-translational modification stabilizes the active conformation of the kinase domain, enabling MEK1 to phosphorylate and activate its only known substrates, ERK1/2, and thereby relay mitogenic, proliferative and differentiation signals from Raf isoforms downstream to diverse cytoplasmic and nuclear targets.

免疫印迹
- WB result of Phospho-MEK1 (Ser298) Recombinant Rabbit mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho-MEK1 (Ser298) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa starve overnight, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43 kDa
  Observed MW: 43 kDa
- WB result of Phospho-MEK1 (Ser298) Recombinant Rabbit mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho-MEK1 (Ser298) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated C6 whole cell lysate 20 µg
  Lane 2: C6 starve overnight, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43 kDa
  Observed MW: 40 kDa
斑点杂交
- Dot blot result of Phospho-MEK1 (Ser298) Recombinant Rabbit mAb
  Lane 1: MEK1 (Ser298) phospho peptide
  Lane 2: MEK1 (Ser298) unmodified peptide
  Primary antibody: Phospho-MEK1 (Ser298) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
- ICC analysis of HeLa cells serum-starvation overnight, then treated with Calyculin A (100 nM, 30 min) (top panel) and untreated HeLa cells (below panel). Anti- Phospho-MEK1 (Ser298) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).