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Phospho-MLKL (Ser345) Recombinant Rabbit mAb (S-2439-64)

Mixed lineage kinase domain-like protein,Mlkl

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6361
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产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-MLKL (Ser345)

  • 分子别名

    Mixed lineage kinase domain-like protein; Mlkl

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane, Cytoplasm, Nucleus

  • Accession

    Q9D2Y4, Q8NB16

  • 克隆号

    S-2439-64

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB

  • 反应种属 ?

    Hu, Ms

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
Dot Blot 1:1000
WB 1:1000 Hu, Ms
背景介绍

  • Phospho-MLKL (Ser345) is the activated, phosphorylated form of the pseudokinase mixed-lineage kinase domain-like protein (MLKL) at serine-345, a critical post-translational modification catalyzed by the necroptotic kinase RIPK3 that triggers MLKL’s oligomerization, translocation to plasma membrane phosphoinositides, and subsequent membrane disruption, thereby executing the terminal, pro-inflammatory cell death pathway known as necroptosis; detection of phospho-MLKL(Ser345) by phospho-specific antibodies thus serves as a specific biomarker for ongoing necroptotic signaling in experimental models and human pathologies including ischemia–reperfusion injury, inflammatory diseases, and certain cancers.

  • 免疫印迹

    • WB result of Phospho-MLKL (Ser345) Recombinant Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: Phospho-MLKL (Ser345) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated L-929 whole cell lysate 20 µg
      Lane 2: L-929 treated with 20 μM Z-VAD for 30 minutes, then treated with 20 ng/ml mTNF-α and 100 nM SM-164 for 4 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 54 kDa
      Observed MW: 54 kDa

  • 斑点杂交

    • Dot blot result of Phospho-NAK/TBK1 (Ser172) Recombinant Rabbit mAb
      Lane 1: MLKL (Ser345) phospho peptide
      Lane 2: MLKL (Ser345) unmodified peptide
      Primary antibody: Phospho-NAK/TBK1 (Ser172) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

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