Phospho-NAK/TBK1 (Ser172) Recombinant Rabbit mAb (S-2481-14)

Serine/threonine-protein kinase TBK1,NF-kappa-B-activating kinase,T2K,TANK-binding kinase 1,NAK,TBK1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6359

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-NAK/TBK1 (Ser172)
分子别名

Serine/threonine-protein kinase TBK1; NF-kappa-B-activating kinase; T2K; TANK-binding kinase 1; NAK; TBK1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

Q9UHD2
克隆号

S-2481-14
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Xe
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000-1:10000 Hu, Ms, Rt
ICC 1:100 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000-1:10000	Hu, Ms, Rt
ICC	1:100	Hu

背景介绍

Phospho-NAK/TBK1 (Ser172) protein is a crucial component in cellular signaling pathways. NAK, also known as TBK1, is a serine/threonine kinase that plays a significant role in innate immune responses and NF-κB activation. When phosphorylated at serine 172, it becomes activated and can phosphorylate downstream substrates such as IRF3, leading to the production of type I interferons. This phosphorylation event at Ser172 is essential for its kinase activity and its involvement in antiviral signaling and inflammatory responses. Additionally, TBK1 has been implicated in autophagy regulation through its interaction with other proteins, further highlighting its multifaceted functions in maintaining cellular homeostasis and responding to various stressors.

免疫印迹
- WB result of Phospho-NAK/TBK1 (Ser172) Recombinant Rabbit mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho-NAK/TBK1 (Ser172) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa starve overnight, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Lane 3: untreated Jurkat whole cell lysate 20 µg
  Lane 4: Jurkat treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 84 kDa
  Observed MW: 84 kDa
- WB result of Phospho-NAK/TBK1 (Ser172) Recombinant Rabbit mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho-NAK/TBK1 (Ser172) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated RAW264.7 whole cell lysate 20 µg
  Lane 2: RAW264.7 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Lane 3: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 4: NIH/3T3 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 84 kDa
  Observed MW: 84 kDa
- WB result of Phospho-NAK/TBK1 (Ser172) Recombinant Rabbit mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho-NAK/TBK1 (Ser172) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated C6 whole cell lysate 20 µg
  Lane 2: C6 starve overnight, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 84 kDa
  Observed MW: 84 kDa
斑点杂交
- Dot blot result of Phospho-NAK/TBK1 (Ser172) Recombinant Rabbit mAb
  Lane 1: NAK/TBK1 (Ser172) phospho peptide
  Lane 2: NAK/TBK1 (Ser172) unmodified peptide
  Primary antibody: Phospho-NAK/TBK1 (Ser172) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
- ICC analysis of HeLa cells serum starvation (overnight), then treated with Calyculin A (100nM, 30 min) (top panel) and untreated HeLa cells (below panel). Anti- Phospho-NAK/TBK1 (Ser172) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).