SFXN1/TCC Rabbit Polyclonal Antibody

Sideroflexin-1,Sfxn1

Datasheet COA

价格￥400.00 （供应商现货： 3-5个工作日）

货号 S0B6358

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

SFXN1/TCC
分子别名

Sideroflexin-1; Sfxn1
免疫原

Recombinant Protein
细胞定位

Mitochondrion
Accession

Q99JR1
抗体类型

Polyclonal antibody
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt, Mk
阳性样本

PC-3, LNCaP, MCF7, HepG2, NIH/3T3, mouse brain, C6, rat brain, COS-7
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt, Mk
IHC-P 1:200 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt, Mk
IHC-P	1:200	Hu, Ms, Rt

背景介绍

SFXN1/TCC (sideroflexin-1/tricarboxylate carrier) is an ~35 kDa integral inner-mitochondrial-membrane protein that functions as a metabolite transporter, exporting mitochondrial acetyl-CoA to the cytosol for lipogenesis and importing serine (and possibly tricarboxylates or iron-related substrates) into the matrix to support one-carbon metabolism, heme/iron–sulfur biogenesis and redox balance, with mutations causing flexed-tail anemia and mitochondrial iron overload in mice and implicating the carrier in human metabolic and neurodegenerative disorders.

免疫印迹
- WB result of SFXN1/TCC Rabbit pAb
  Primary antibody: SFXN1/TCC Rabbit pAb at 1/1000 dilution
  Lane 1: PC-3 whole cell lysate 20 µg
  Lane 2: LNCaP whole cell lysate 20 µg
  Lane 3: MCF7 whole cell lysate 20 µg
  Lane 4: HepG2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 35 kDa
  Observed MW: 32 kDa
- WB result of SFXN1/TCC Rabbit pAb
  Primary antibody: SFXN1/TCC Rabbit pAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 35 kDa
  Observed MW: 32 kDa
- WB result of SFXN1/TCC Rabbit pAb
  Primary antibody: SFXN1/TCC Rabbit pAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 35 kDa
  Observed MW: 32 kDa
- WB result of SFXN1/TCC Rabbit pAb
  Primary antibody: SFXN1/TCC Rabbit pAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 35 kDa
  Observed MW: 32 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SFXN1/TCC antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti-SFXN1/TCC antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-SFXN1/TCC antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-SFXN1/TCC antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-SFXN1/TCC antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-SFXN1/TCC antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of SFXN1/TCC in tumor tissue.
- Expression of SFXN1/TCC in human tissue.
- Expression of SFXN1/TCC in mouse & rat tissue.