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Rabbit抗原名称
Connexin 43/GJA1分子别名
Gap junction alpha-1 protein; Connexin-43 (Cx43); Gap junction 43 kDa heart protein; GJAL免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P17302抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
mouse heart, rat heart纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Ms, Rt IHC-P 1:500-1:1000 Hu, Ms, Rt IF 1:100 Ms, Rt
Connexin 43 (Cx43), also known as GJA1, is the predominant gap junction protein in vertebrates, encoded by the GJA1 gene. It assembles into hexameric structures in the Golgi apparatus and translocates to the plasma membrane to form gap junction channels, facilitating intercellular communication by allowing the rapid transmission of ions and small molecules between adjacent cells. Cx43 is widely expressed in various tissues, including the heart, where it plays a crucial role in cardiac function and protection against arrhythmias. In addition to its canonical role in gap junction formation, Cx43 has been implicated in non-canonical functions such as cell proliferation, tumor suppression, and stress response. For example, the alternatively translated isoform GJA1-20k protects Cx43 from degradation, stabilizes actin, and mediates ischemic preconditioning. Dysregulation of Cx43 expression is associated with various diseases, including cardiac arrhythmias, cancer, and neurodegenerative disorders.
免疫印迹
WB result of Connexin 43 / GJA1 Rabbit pAb
Primary antibody: Connexin 43 / GJA1 Rabbit pAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 38, 39 kDa
This blot was developed with high sensitivity substrateWB result of Connexin 43 / GJA1 Rabbit pAb
Primary antibody: Connexin 43 / GJA1 Rabbit pAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 38, 39 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Connexin 43 / GJA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-Connexin 43 / GJA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-Connexin 43 / GJA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-Connexin 43 / GJA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat kidney. Anti-Connexin 43 / GJA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-Connexin 43 / GJA1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat cardiac muscle. Anti-Connexin 43 / GJA1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Connexin 43 / GJA1 in tumor tissue.
Expression of Connexin 43 / GJA1 in human tissue.
Expression of Connexin 43 / GJA1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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