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ERAP1 Recombinant Rabbit mAb (S-2509-38)

Endoplasmic reticulum aminopeptidase 1,ARTS-1,Adipocyte-derived leucine aminopeptidase (A-LAP),Aminopeptidase PILS,Puromycin-insensitive leucyl-specific aminopeptidase (PILS-AP),Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator,APPILS,ARTS1,KIAA0525

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6354

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

ERAP1
分子别名

Endoplasmic reticulum aminopeptidase 1; ARTS-1; Adipocyte-derived leucine aminopeptidase (A-LAP); Aminopeptidase PILS; Puromycin-insensitive leucyl-specific aminopeptidase (PILS-AP); Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator; APPILS; ARTS1; KIAA0525
免疫原

Synthetic Peptide
细胞定位

Endoplasmic reticulum
Accession

Q9NZ08
克隆号

S-2509-38
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

K562, A431, HDLM-2, HUVEC, Jurkat, HepG2, Ramos, mouse liver, mouse heart
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms
IHC-P 1:2000 Hu, Ms, Rt
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms
IHC-P	1:2000	Hu, Ms, Rt
ICC	1:500	Hu

背景介绍

ERAP1 (Endoplasmic Reticulum Aminopeptidase 1) is a zinc-dependent metalloaminopeptidase localized in the endoplasmic reticulum that plays a critical role in antigen processing by trimming N-terminal residues of peptides to optimize their length (8–10 amino acids) for loading onto MHC class I molecules, thereby shaping the immunopeptidome and influencing immune responses; it also modulates inflammation by cleaving cytokine receptors and is genetically associated with autoimmune diseases such as ankylosing spondylitis and Behçet’s disease, particularly in individuals carrying specific HLA alleles like HLA-B27 or HLA-B*51.

免疫印迹
- WB result of ERAP1 Recombinant Rabbit mAb
  Primary antibody: ERAP1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled K562 whole cell lysate 20 µg
  Lane 2: unboiled A431 whole cell lysate 20 µg
  Lane 3: unboiled HDLM-2 whole cell lysate 20 µg
  Lane 4: unboiled HUVEC whole cell lysate 20 µg
  Lane 5: unboiled Jurkat whole cell lysate 20 µg
  Lane 6: unboiled HepG2 whole cell lysate 20 µg
  Lane 7: unboiled Ramos whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 107 kDa
  Observed MW: 110 kDa
- WB result of ERAP1 Recombinant Rabbit mAb
  Primary antibody: ERAP1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled mouse liver lysate 20 µg
  Lane 2: unboiled mouse heart lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 107 kDa
  Observed MW: 110 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human liver. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human placenta. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse liver. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat liver. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in A431 cells. Anti- ERAP1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of ERAP1 in tumor tissue.
- Expression of ERAP1 in human tissue.
- Expression of ERAP1 in mouse & rat tissue.