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Rabbit抗原名称
VDAC2分子别名
Non-selective voltage-gated ion channel VDAC2; VDAC-2; hVDAC2; Outer mitochondrial membrane protein porin 2免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
P45880抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
COS-7, HEK-293, HepG2, Jurkat, RAW264.7, mouse brain, PC-12, rat heart预测反应种属
(反应种属缩写表)Hm, Rb, Pg, Bv, Tk纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:200 Hu, Ms, Rt
VDAC2 (Voltage-Dependent Anion Channel 2) is an evolutionarily conserved, ~32 kDa integral β-barrel porin embedded in the outer mitochondrial membrane where it orchestrates the flux of ATP, ADP, metabolites, and ions between the cytosol and mitochondria, serves as a crucial docking platform for pro- and anti-apoptotic Bcl-2 family proteins to modulate cytochrome-c release and intrinsic apoptosis, interacts with hexokinases to couple glycolysis to oxidative phosphorylation in cancer metabolism, and responds to transmembrane potential and pharmacological effectors such as erastin in ferroptosis; its expression is nearly ubiquitous yet highest in heart, skeletal muscle, and brain, and dysregulation contributes to neurodegeneration, cardiomyopathy, and chemoresistance, making VDAC2 a convergent therapeutic target for metabolic reprogramming and cell-death modulation.
免疫印迹
WB result of VDAC2 Rabbit pAb
Primary antibody: VDAC2 Rabbit pAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDaWB result of VDAC2 Rabbit pAb
Primary antibody: VDAC2 Rabbit pAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDaWB result of VDAC2 Rabbit pAb
Primary antibody: VDAC2 Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDaWB result of VDAC2 Rabbit pAb
Primary antibody: VDAC2 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-VDAC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-VDAC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-VDAC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-VDAC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-VDAC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of VDAC2 in tumor tissue.
Expression of VDAC2 in human tissue.
Expression of VDAC2 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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