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Rabbit抗原名称
Phospho-RIP3 (Thr231/Ser232)分子别名
Receptor-interacting serine/threonine-protein kinase 3; RIP-like protein kinase 3; Receptor-interacting protein 3 (RIP-3; mRIP3); Rip3; Ripk3细胞定位
Cytoplasm, NucleusAccession
Q9QZL0克隆号
S-3487抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Ms纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Ms
Phospho-RIP3 (Thr231/Ser232) is the phosphorylated, catalytically active form of the 57 kDa serine/threonine kinase Receptor-Interacting Protein 3 (RIP3, RIPK3); dual phosphorylation at Thr231 and Ser232 within the conserved activation loop is induced by TNF-α, TRAIL or viral infection, converting RIP3 into a core driver of necroptosis—a programmed, caspase-independent inflammatory cell death pathway—via autophosphorylation and subsequent phosphorylation of downstream substrates such as MLKL, thereby assembling the necrosome complex and triggering membrane rupture, calcium influx and damage-associated molecular pattern (DAMP) release; detection of Phospho-RIP3 (Thr231/Ser232) by phospho-specific antibodies is therefore a key biomarker for necroptotic signaling in cancer, ischemia-reperfusion injury and inflammatory diseases.
免疫印迹
WB result of Phospho-RIP3 (Thr231/Ser232) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-RIP3 (Thr231/Ser232) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated L-929 whole cell lysate 20 µg
Lane 2: L-929 treated with 20 μM Z-VAD for 30 minutes, then treated with 20 ng/ml mTNF-α and 100 nM SM-164 for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 54 kDa
This blot was developed with high sensitivity substrate
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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