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Rabbit抗原名称
LRRC15分子别名
Leucine-rich repeat-containing protein 15; Leucine-rich repeat protein induced by beta-amyloid homolog (hLib); LIB免疫原
Recombinant Protein细胞定位
Cell membraneAccession
Q8TF66-1克隆号
S-2473-43抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
U-87 MG, Saos-2纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:2000 Hu ICC 1:500 Hu ICFCM 1:50 Hu
LRRC15 (leucine-rich repeat-containing protein 15) is a 581-amino-acid type I transmembrane receptor encoded on chromosome 3q29, featuring 15 extracellular leucine-rich repeats that fold into a horseshoe-shaped ligand-binding domain connected to a single-pass membrane helix and a short 22-residue cytoplasmic tail, and it is physiologically expressed at low levels in placenta, skin, tonsil and gastric mucosa but becomes strongly up-regulated by TGF-β and IL-1β in cancer-associated fibroblasts, wound-healing myofibroblasts and virally inflamed lungs, where it engages extracellular matrix proteins such as fibronectin, collagen and laminin to modulate cell adhesion, migration and stemness, acts as a decoy receptor that sequesters SARS-CoV-2 spike protein without mediating viral entry thereby exerting antiviral and antifibrotic effects, and promotes tumor progression and metastasis through integrin-β1/FAK/PI3K signaling, making LRRC15 a promising stromal and mesenchymal biomarker and a therapeutic target for antibody–drug conjugates and small-molecule inhibitors in cancer, fibrosis and infectious diseases .
免疫印迹
WB result of LRRC15 Recombinant Rabbit mAb
Primary antibody: LRRC15 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Lane 3: Saos-2 whole cell lysate 20 µg
Negative control: Daudi whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 60-90 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Daudi (Human Burkitt's lymphoma lymphoblast, Left) / U-87 MG (Human glioblastoma-astrocytoma epithelial cell, Right) labelling LRRC15 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: Daudi
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-LRRC15 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-LRRC15 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-LRRC15 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-LRRC15 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-LRRC15 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-LRRC15 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human osteosarcoma. Anti-LRRC15 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human synovial sarcoma. Anti-LRRC15 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in U87-MG cells (top panel) and negative staining in Daudi cells (below panel). Anti-LRRC15 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of LRRC15 in tumor tissue.
Expression of LRRC15 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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