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Rabbit抗原名称
LPA分子别名
Apolipoprotein(a); Apo(a); Lp(a); LPA免疫原
Recombinant ProteinAccession
P08519抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
human serum, human plasma纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:4000 Hu IF 1:500 Hu
LPA protein refers to apolipoprotein(a), the distinctive glycoprotein covalently linked via a single disulfide bond to apolipoprotein B-100 on a low-density lipoprotein (LDL)-like particle to form lipoprotein(a) [Lp(a)], a liver-synthesized macromolecule whose circulating concentration—ranging from undetectable to >100 mg/dL and varying more than 1,000-fold among individuals—is almost entirely dictated by the LPA gene’s size polymorphism of kringle IV repeats, rendering levels largely fixed from early childhood and minimally responsive to diet, lifestyle, or standard LDL-lowering therapies. Beyond its structural role, apo(a)’s striking sequence homology to plasminogen imparts anti-fibrinolytic and pro-thrombotic properties, while its capacity to bind oxidized phospholipids and promote macrophage lipid uptake confers potent atherogenicity, making elevated Lp(a) an independent, causal risk factor for atherosclerotic cardiovascular disease, calcific aortic stenosis, and thrombo-embolic events, although its precise physiological function remains elusive.
免疫印迹
WB result of LPA Rabbit pAb
Primary antibody: LPA Rabbit pAb at 1/1000 dilution
Lane 1: human serum lysate 5 µg
Lane 2: human plasma lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG(H+L) Recombinant pAb (min X Hu Sr Prot), HRP conjugated at 1/10000 dilution
Predicted MW: 226 kDa
Observed MW: 110-500 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-LPA antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-LPA antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-LPA antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human glioma. Anti-LPA antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human ovarian cancer. Anti-LPA antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human liver. Anti-LPA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of LPA in tumor tissue.
Expression of LPA in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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