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Rabbit抗原名称
IRGQ分子别名
Immunity-related GTPase family Q protein; IRGQ1免疫原
Synthetic Peptide细胞定位
LysosomeAccession
Q8WZA9克隆号
S-2648-17抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Mk阳性样本
RD, SK-BR-3, MCF7, BxPC-3, U-2 OS, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Mk IHC-P 1:200 Hu, Ms
IRGQ (Immunity-related GTPase family Q protein) is a newly recognized autophagy receptor that orchestrates quality control of MHC class I molecules by binding to the autophagy proteins GABARAPL2 and LC3B, thereby routing misfolded MHC-I heavy chains to lysosomal degradation; its absence allows these defective chains to accumulate and reach the cell surface, enhancing CD8⁺ T cell responses and improving survival in hepatocellular carcinoma models, and IRGQ is also implicated in Alzheime’s pathogenesis and exhibits broad cytoplasmic/nuclear expression across human tissues.
免疫印迹
WB result of IRGQ Recombinant Rabbit mAb
Primary antibody: IRGQ Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RD whole cell lysate 20 µg
Lane 2: SK-BR-3 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: BxPC-3 whole cell lysate 20 µg
Lane 5: U-2 OS whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 65 kDaWB result of IRGQ Recombinant Rabbit mAb
Primary antibody: IRGQ Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 65 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-IRGQ antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-IRGQ antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-IRGQ antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-IRGQ antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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