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Hint1 Recombinant Rabbit mAb (S-2693-67)

Adenosine 5'-monophosphoramidase HINT1,Desumoylating isopeptidase HINT1,Histidine triad nucleotide-binding protein 1,Protein kinase C inhibitor 1,Protein kinase C-interacting protein 1 (PKCI-1),HINT,PKCI1,PRKCNH1,HINT1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6339

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Hint1
分子别名

Adenosine 5'-monophosphoramidase HINT1; Desumoylating isopeptidase HINT1; Histidine triad nucleotide-binding protein 1; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1 (PKCI-1); HINT; PKCI1; PRKCNH1; HINT1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

P49773
克隆号

S-2693-67
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

Ramos, HeLa, 293T, MCF7, HepG2, Jurkat, mouse brain, rat liver, COS-7
预测反应种属
(反应种属缩写表)

Bv, Rb
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:2000 Hu, Ms, Rt, Mk
IHC-P 1:1000 Hu, Ms, Rt
ICC 1:100 Hu
ICFCM 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000-1:2000	Hu, Ms, Rt, Mk
IHC-P	1:1000	Hu, Ms, Rt
ICC	1:100	Hu
ICFCM	1:500	Hu

背景介绍

HINT1 (histidine triad nucleotide-binding protein 1) is a ubiquitously expressed, 13.7 kDa zinc-binding homodimeric enzyme that hydrolyzes purine nucleotide phosphoramidates via a double-displacement mechanism while acting as a multifunctional scaffold; it couples μ-opioid and NMDA receptors to tune analgesia and addiction behavior, suppresses tumorigenesis by inhibiting MITF and β-catenin, and its loss-of-function mutations cause autosomal recessive axonal neuropathy with neuromyotonia, making it a key player in neuropsychiatric disorders, pain regulation, and cancer.

免疫印迹
- WB result of Hint1 Recombinant Rabbit mAb
  Primary antibody: Hint1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Ramos whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: 293T whole cell lysate 20 µg
  Lane 4: MCF7 whole cell lysate 20 µg
  Lane 5: HepG2 whole cell lysate 20 µg
  Lane 6: Jurkat whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 14 kDa
  Observed MW: 15 kDa
- WB result of Hint1 Recombinant Rabbit mAb
  Primary antibody: Hint1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 14 kDa
  Observed MW: 16 kDa
- WB result of Hint1 Recombinant Rabbit mAb
  Primary antibody: Hint1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 14 kDa
  Observed MW: 16 kDa
- WB result of Hint1 Recombinant Rabbit mAb
  Primary antibody: Hint1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 14 kDa
  Observed MW: 15 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell) labelling Hint1 antibody at 1/500 dilution (0.1 μg)/ (red) compared with a Rabbit monoclonal IgG (black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-Hint1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Hint1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Hint1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse colon. Anti-Hint1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat stomach. Anti-Hint1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in MCF7 cells. Anti- Hint1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of Hint1 in tumor tissue.
- Expression of Hint1 in human tissue.
- Expression of Hint1 in mouse & rat tissue.