APRIL Recombinant Rabbit mAb (S-2667-23)
Tumor necrosis factor ligand superfamily member 13,A proliferation-inducing ligand (APRIL),TNF- and APOL-related leukocyte expressed ligand 2 (TALL-2),TNF-related death ligand 1 (TRDL-1),CD256,TALL2,ZTNF2,TNFSF13
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宿主来源
Rabbit抗原名称
APRIL分子别名
Tumor necrosis factor ligand superfamily member 13; A proliferation-inducing ligand (APRIL); TNF- and APOL-related leukocyte expressed ligand 2 (TALL-2); TNF-related death ligand 1 (TRDL-1); CD256; TALL2; ZTNF2; TNFSF13免疫原
Synthetic Peptide细胞定位
SecretedAccession
O75888克隆号
S-2667-23抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Rt阳性样本
Raji, HeLa, COLO 205, HT-29, PC-12纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu, Rt
A proliferation-inducing ligand (APRIL), officially designated CD256 and encoded by the TNFSF13 gene, is a type-II transmembrane protein of the tumor necrosis factor (TNF) superfamily that is cleaved into a soluble cytokine; it binds the receptors BCMA and TACI on B cells, thereby driving B-cell maturation, plasma-cell survival in the bone marrow, and class-switch recombination, while also promoting tumor-cell proliferation in multiple myeloma and other B-cell malignancies, and is being pursued therapeutically via neutralizing antibodies or decoy receptors to curb autoimmunity and cancer .
免疫印迹
WB result of APRIL Recombinant Rabbit mAb
Primary antibody: APRIL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: COLO 205 whole cell lysate 20 µg
Lane 4: HT-29 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 29 kDaWB result of APRIL Recombinant Rabbit mAb
Primary antibody: APRIL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 29 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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