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Rabbit抗原名称
CCL4分子别名
C-C motif chemokine 4; G-26 T-lymphocyte-secreted protein; HC21; Lymphocyte activation gene 1 protein (LAG-1); MIP-1-beta(1-69); Macrophage inflammatory protein 1-beta (MIP-1-beta); LAG1; MIP1B; SCYA4免疫原
Recombinant Protein细胞定位
SecretedAccession
P13236克隆号
S-2174-23抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu ICC 1:500 Hu
CCL4, also known as macrophage inflammatory protein-1β (MIP-1β), is an 8 kDa chemokine that belongs to the CC subfamily and is primarily secreted by activated T cells, macrophages, dendritic cells, and NK cells to orchestrate immune responses by binding to the G-protein-coupled receptors CCR5 and CCR8, thereby directing chemotaxis of monocytes, NK cells, and various T-cell subsets to sites of infection or inflammation, while also contributing to the pathogenesis of inflammatory diseases such as rheumatoid arthritis, HIV infection—where it competes with the virus for CCR5 binding—and certain cancers, and is further characterized by a compact tertiary structure stabilized by two disulfide bridges between conserved cysteine residues, a gene located on human chromosome 17q12, and tight regulation at transcriptional and post-transcriptional levels by cytokines like TNF-α and IL-1β.
免疫印迹
WB result of CCL4 Recombinant Rabbit mAb
Primary antibody: CCL4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/ml LPS for 4 hours and then add 1 ug/ml Brefeldin A for the last 3 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 10 kDa
Observed MW: 12 kDa
免疫细胞化学
ICC analysis of THP-1 cells treated with PMA (100nM, overnight), then with LPS(100ng/ml,4h), followed by adding Brefeldin A (1ug/ml, 3h) (top panel) and untreated THP-1 cells (below panel). Anti- CCL4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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