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CYP3A4 Recombinant Rabbit mAb (S-2281-146)

Cytochrome P450 3A4,1,4-cineole 2-exo-monooxygenase,8-cineole 2-exo-monooxygenase,Albendazole monooxygenase (sulfoxide-forming),Albendazole sulfoxidase,CYPIIIA3,CYP3A3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6328

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

CYP3A4
分子别名

Cytochrome P450 3A4; 1,4-cineole 2-exo-monooxygenase; 1,8-cineole 2-exo-monooxygenase; Albendazole monooxygenase (sulfoxide-forming); Albendazole sulfoxidase; CYPIIIA3; CYP3A3
免疫原

Synthetic Peptide
细胞定位

Endoplasmic reticulum
Accession

P08684
克隆号

S-2281-146
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
阳性样本

mouse liver
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IF ?
稀释度

应用稀释度推荐种属

WB 1:1000 Ms
IHC-P 1:2000 Hu, Ms
IF 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Ms
IHC-P	1:2000	Hu, Ms
IF	1:500	Hu

背景介绍

CYP3A4 is a 57-kDa cytochrome P450 mono-oxygenase anchored in the endoplasmic reticulum of human liver and intestine, where its large, highly lipophilic active site binds a broad spectrum of substrates—including more than half of all marketed drugs—catalyzing the insertion of an oxygen atom into C–H or C=C bonds to increase polarity and facilitate elimination; expression is up-regulated by nuclear receptor PXR and down-regulated by inflammatory cytokines, leading to up to 40-fold inter-individual variability, and common genetic variants (e.g., *22) modestly alter activity, while potent inhibitors like ketoconazole or grapefruit furanocoumarins and inducers like rifampicin or St. John’s wort can precipitate clinical drug–drug interactions, making CYP3A4 a central determinant of systemic exposure, efficacy, and toxicity for statins, calcium-channel blockers, immunosuppressants, and many oncology agents.

免疫印迹
- WB result of CYP3A4 Recombinant Rabbit mAb
  Primary antibody: CYP3A4 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse brain lysate 20 µg
  Lane 2: mouse liver lysate 20 µg
  Negative control: mouse brain lysate
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 57 kDa
  Observed MW: 50 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human liver. Anti-CYP3A4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CYP3A4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse liver. Anti-CYP3A4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
免疫荧光
- IF shows positive staining in paraffin-embedded human liver. Anti-CYP3A4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
- Expression of CYP3A4 in tumor tissue.
- Expression of CYP3A4 in human tissue.