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EHMT2/G9A Recombinant Rabbit mAb (S-2232-3)

Histone-lysine N-methyltransferase EHMT2,Euchromatic histone-lysine N-methyltransferase 2,HLA-B-associated transcript 8,Histone H3-K9 methyltransferase 3 (H3-K9-HMTase 3),Lysine N-methyltransferase 1C,Protein G9a,BAT8,C6orf30,KMT1C,NG36

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6326

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

EHMT2/G9A
分子别名

Histone-lysine N-methyltransferase EHMT2; Euchromatic histone-lysine N-methyltransferase 2; HLA-B-associated transcript 8; Histone H3-K9 methyltransferase 3 (H3-K9-HMTase 3); Lysine N-methyltransferase 1C; Protein G9a; BAT8; C6orf30; KMT1C; NG36
免疫原

Recombinant Protein
细胞定位

Nucleus
Accession

Q96KQ7
克隆号

S-2232-3
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

293T, HeLa, HepG2, Jurkat, A431, NIH/3T3, RAW264.7, C6
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu, Ms
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu, Ms
ChIP	1:20-1:50	Hu

背景介绍

EHMT2 (also called G9a) is a nuclear lysine methyltransferase that chiefly di-methylates histone H3 at lysine 9 (H3K9me2) and lysine 27 (H3K27me1/2) to establish facultative heterochromatin and transcriptionally silence target genes, and it can act either as a repressor or, through auto-methylation-dependent recruitment of cofactors such as HP1γ and p300, as a co-activator of transcription; the enzyme exists as a homodimer or heterodimer with the related GLP/EHMT1, contains an N-terminal ankyrin-repeat domain for chromatin docking and a catalytic SET domain for methyl transfer, and its dysregulation—via overexpression in diverse solid and hematologic cancers or down-regulation in addiction and neuropathic pain—alters the expression of hundreds of genes by both histone and non-histone methylation, thereby controlling embryonic development, stem-cell lineage commitment, immune-cell differentiation, synaptic remodeling, and tumorigenicity .

免疫印迹
- WB result of EHMT2/G9A Recombinant Rabbit mAb
  Primary antibody: EHMT2/G9A Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: 293T whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: HepG2 whole cell lysate 20 µg
  Lane 4: Jurkat whole cell lysate 20 µg
  Lane 5: A431 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 132 kDa
  Observed MW: 140-180 kDa
- WB result of EHMT2/G9A Recombinant Rabbit mAb
  Primary antibody: EHMT2/G9A Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: RAW264.7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 132 kDa
  Observed MW: 140-180 kDa
- WB result of EHMT2/G9A Recombinant Rabbit mAb
  Primary antibody: EHMT2/G9A Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 132 kDa
  Observed MW: 140-180 kDa
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- EHMT2/G9A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti- EHMT2/G9A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used EHMT2/G9A Recombinant Rabbit mAb (S-2232-3) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation. Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  
  qPCR showed the enrichment of VRK3 and SAT-α in EHMT2/G9A
  Recombinant Rabbit mAb (S-2232-3)-immunoprecipitated sample.