所有图片（1/6）

JNK1 + JNK2 + JNK3 Recombinant Rabbit mAb (S-3385)

Mitogen-activated protein kinase 8,MAP kinase 8,MAPK 8,JNK-46,Stress-activated protein kinase 1c (SAPK1c),Stress-activated protein kinase JNK1,c-Jun N-terminal kinase 1,PRKM8,SAPK1,SAPK1C,MAPK8,Mitogen-activated protein kinase 9,MAP kinase 9,MAPK 9,JNK-55,Stress-activated protein kinase 1a (SAPK1a),Stress-activated protein kinase JNK2,c-Jun N-terminal kinase 2,MAPK9,PRKM9,SAPK1A,Mitogen-activated protein kinase 10,MAP kinase 10,MAPK 10,MAP kinase p49 3F12,Stress-activated protein kinase 1b (SAPK1b),Stress-activated protein kinase JNK3,c-Jun N-terminal kinase 3,MAPK10,JNK3A,PRKM10,SAPK1B

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6321

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

JNK1 + JNK2 + JNK3
分子别名

Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c (SAPK1c); Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; PRKM8; SAPK1; SAPK1C; MAPK8; Mitogen-activated protein kinase 9; MAP kinase 9; MAPK 9; JNK-55; Stress-activated protein kinase 1a (SAPK1a); Stress-activated protein kinase JNK2; c-Jun N-terminal kinase 2; MAPK9; PRKM9; SAPK1A; Mitogen-activated protein kinase 10; MAP kinase 10; MAPK 10; MAP kinase p49 3F12; Stress-activated protein kinase 1b (SAPK1b); Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3; MAPK10; JNK3A; PRKM10; SAPK1B
细胞定位

Nucleus, Synapse, Cytoplasm
Accession

P45983, P45984, P53779
克隆号

S-3385
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt, Zf
阳性样本

K562, HeLa, Jurkat, MCF7, Neuro-2a, RAW264.7, PC-12, C6, Zebrafsih
预测反应种属
(反应种属缩写表)

Ck, Bv, Dg, AfGrMk, Xe
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt, Zf
ICC 1:500 Hu
ICFCM 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt, Zf
ICC	1:500	Hu
ICFCM	1:50	Hu

背景介绍

JNK1, JNK2, and JNK3 are the three main isoforms of the c-Jun N-terminal Kinase family, which belongs to the MAPK (Mitogen-Activated Protein Kinase) superfamily. They are critical signaling molecules that translate extracellular stimuli into intracellular responses. Encoded by distinct genes (MAPK8, MAPK9, and MAPK10, respectively), these three isoforms share high structural similarity but also exhibit significant functional differences: JNK1 and JNK2 are ubiquitously expressed across various tissues and are involved in regulating core cellular processes in response to stresses such as oxidative stress and genotoxic stress, as well as inflammatory cytokines. These processes include cell proliferation, differentiation, apoptosis, and metabolic homeostasis. In contrast, the expression of JNK3 is primarily restricted to the brain, heart, and testes, with particularly high abundance in neurons. This localized expression assigns JNK3 a central role in central nervous system-specific functions, such as regulating neuronal apoptosis and synaptic plasticity, and it is closely implicated in the pathology of various neurodegenerative diseases like Alzheimer's and Parkinson's. Although they share common substrates, such as the transcription factor c-Jun (which they activate by phosphorylating its N-terminus), subtle differences in their promoter activity, binding preferences for scaffold proteins, and subcellular localization often lead to unique, and sometimes opposing, functions in specific contextual settings. This intricate interplay forms a sophisticated signaling network that plays a vital role in determining cell fate and the organism's response to stress.

免疫印迹
- WB result of JNK1 + JNK2 + JNK3 Recombinant Rabbit mAb
  Primary antibody: JNK1 + JNK2 + JNK3 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: K562 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Lane 4: MCF7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48, 53 kDa
  Observed MW: 40, 53 kDa
- WB result of JNK1 + JNK2 + JNK3 Recombinant Rabbit mAb
  Primary antibody: JNK1 + JNK2 + JNK3 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Neuro-2a whole cell lysate 20 µg
  Lane 2: RAW264.7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48, 53 kDa
  Observed MW: 40, 53 kDa
- WB result of JNK1 + JNK2 + JNK3 Recombinant Rabbit mAb
  Primary antibody: JNK1 + JNK2 + JNK3 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48, 53 kDa
  Observed MW: 40, 53 kDa
- WB result of JNK1 + JNK2 + JNK3 Recombinant Rabbit mAb
  Primary antibody: JNK1 + JNK2 + JNK3 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: zebrafish lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48, 53 kDa
  Observed MW: 40, 65 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling JNK1 + JNK2 + JNK3 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- JNK1 + JNK2 + JNK3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).