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KDM4A Recombinant Rabbit mAb (S-957-138)

Lysine-specific demethylase 4A,JmjC domain-containing histone demethylation protein 3A,Jumonji domain-containing protein 2A,[histone H3]-trimethyl-L-lysine(36) demethylase 4A,[histone H3]-trimethyl-L-lysine(9) demethylase 4A,JHDM3A,JMJD2,JMJD2A,KIAA0677

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6320

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

KDM4A
分子别名

Lysine-specific demethylase 4A; JmjC domain-containing histone demethylation protein 3A; Jumonji domain-containing protein 2A; [histone H3]-trimethyl-L-lysine(36) demethylase 4A; [histone H3]-trimethyl-L-lysine(9) demethylase 4A; JHDM3A; JMJD2; JMJD2A; KIAA0677
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

O75164
克隆号

S-957-138
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HEK-293, LNCaP, MCF7, HeLa, SH-SY5Y, NIH/3T3, PC-12
预测反应种属
(反应种属缩写表)

Or
纯化方式

Protein A
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:4000 Hu, Ms, Rt
IHC-P 1:100 Hu, Ms, Rt
ICC 1:1000-1:2000 Hu, Ms

应用	稀释度	推荐种属
WB	1:1000-1:4000	Hu, Ms, Rt
IHC-P	1:100	Hu, Ms, Rt
ICC	1:1000-1:2000	Hu, Ms

背景介绍

KDM4A (also known as JMJD2A, JHDM3A) is a 125 kDa Fe(II)- and α-ketoglutarate-dependent nuclear histone demethylase that specifically removes di- and tri-methyl marks from H3K9 and H3K36, thereby acting as a transcriptional repressor or activator, depending on context, to modulate chromatin structure and gene expression; it cycles in abundance through G1/S to G2/M via ubiquitin-proteasome control, localizes to nucleoli where it associates with rDNA and RNA polymerase I, participates in replication timing and site-specific DNA re-replication, and also localizes to centrosomes where its enzymatic activity safeguards centrosome integrity and mitotic fidelity, while its dysregulation is linked to diverse cancers and other diseases, making it a therapeutic and diagnostic target.

免疫印迹
- WB result of KDM4A Recombinant Rabbit mAb
  Primary antibody: KDM4A Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NCCIT whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 120 kDa
  Observed MW: 150 kDa
- WB result of KDM4A Recombinant Rabbit mAb
  Primary antibody: KDM4A Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: LNCaP whole cell lysate 20 µg
  Lane 3: MCF7 whole cell lysate 20 µg
  Lane 4: HeLa whole cell lysate 20 µg
  Lane 5: SH-SY5Y whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 120 kDa
  Observed MW: 150 kDa
  This blot was developed with high sensitivity substrate
- WB result of KDM4A Recombinant Rabbit mAb
  Primary antibody: KDM4A Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 120 kDa
  Observed MW: 150 kDa
  This blot was developed with high sensitivity substrate
- WB result of KDM4A Recombinant Rabbit mAb
  Primary antibody: KDM4A Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 120 kDa
  Observed MW: 150 kDa
  This blot was developed with high sensitivity substrate
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-KDM4A antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon. Anti-KDM4A antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-KDM4A antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-KDM4A antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti-KDM4A antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti-KDM4A antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in NCCIT cells. Anti-KDM4A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti-KDM4A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of KDM4A in tumor tissue.
- Expression of KDM4A in human tissue.
- Expression of KDM4A in mouse & rat tissue.