NTRK1/TrkA Recombinant Rabbit mAb (S-2524-16)
High affinity nerve growth factor receptor,Neurotrophic tyrosine kinase receptor type 1,TRK1-transforming tyrosine kinase protein,Tropomyosin-related kinase A,Tyrosine kinase receptor,Tyrosine kinase receptor A (Trk-A),MTC,TRK,TRKA
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宿主来源
Rabbit抗原名称
NTRK1/TrkA分子别名
High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A (Trk-A); MTC; TRK; TRKA免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P04629克隆号
S-2524-16抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
TF-1, K562, SH-SY5Y纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu
NTRK1/TrkA, encoded on chromosome 1q23.1, is a 140 kDa high-affinity receptor tyrosine kinase for nerve growth factor (NGF) that, upon ligand-induced dimerization and autophosphorylation at Tyr490, Tyr751 and Tyr785, activates the PI3K/Akt, Ras/MAPK and PLCγ pathways to orchestrate neuronal survival, differentiation, nociception and evasion of apoptosis, while its fusion proteins or mutations can drive oncogenesis and are targeted by TRK inhibitors.
免疫印迹
WB result of NTRK1/TrkA Recombinant Rabbit mAb
Primary antibody: NTRK1/TrkA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: TF-1 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Negative control: 293T whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 140 kDa
This blot was developed with high sensitivity substrate
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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