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Rabbit抗原名称
Phospho-Bcl-2 (Ser70)分子别名
Apoptosis regulator Bcl-2; BCL2免疫原
Synthetic Peptide细胞定位
Nucleus, Mitochondrion, Endoplasmic reticulum, CytoplasmAccession
P10415克隆号
S-1344-257抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
Phospho-Bcl-2 (Ser70) is the B-cell lymphoma 2 (Bcl-2) protein bearing a phosphate group on serine-70, a post-translational modification that dynamically tunes its anti-apoptotic function by altering its interaction with pro-apoptotic BH3-only proteins and modulating mitochondrial outer-membrane permeabilization, thereby serving as a key regulatory switch in intrinsic apoptosis signaling and a biomarker for chemoresistance in hematologic and solid tumors.
免疫印迹
WB result of Phospho-Bcl-2 (Ser70) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Bcl-2 (Ser70) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Jurkat whole cell lysate 20 µg
Lane 2: Jurkat treated with 1 µM paclitaxel for 24 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 25 kDaWB result of Phospho-Bcl-2 (Ser70) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Bcl-2 (Ser70) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat treated with 1 µM paclitaxel for 24 hours whole cell lysate 20 µg
Lane 2: Jurkat treated with 1 µM paclitaxel for 24 hours, then treated with phosphatase whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 25 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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