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PRMT5 Recombinant Rabbit mAb (S-2336-126)

Protein arginine N-methyltransferase 5,72 kDa ICln-binding protein,Histone-arginine N-methyltransferase PRMT5,Jak-binding protein 1,Shk1 kinase-binding protein 1 homolog (SKB1 homolog,SKB1Hs),HRMT1L5,IBP72,JBP1,SKB1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6308

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

PRMT5
分子别名

Protein arginine N-methyltransferase 5; 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog (SKB1 homolog; SKB1Hs); HRMT1L5; IBP72; JBP1; SKB1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

O14744
克隆号

S-2336-126
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
阳性样本

HeLa, HEK-293, HepG2, C2C12
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20°C as supplied
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms
IHC-P 1:1000 Hu
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms
IHC-P	1:1000	Hu
ICC	1:500	Hu

背景介绍

PRMT5 (protein arginine methyltransferase 5) is an evolutionarily conserved type II methyltransferase that catalyzes the symmetric dimethylation of arginine residues (SDMA) on histones H3R8me2s and H4R3me2s and on a broad spectrum of non-histone substrates including Sm proteins, RNA-binding proteins and transcription factors, thereby orchestrating chromatin remodeling, spliceosome assembly, transcriptional repression, signal transduction and stem-cell pluripotency; it functions as a tetrameric complex with MEP50, localizes to both cytoplasm and nucleus, exhibits oncogenic addiction in cancers such as mantle cell lymphoma and glioblastoma through its control of methionine and serine metabolism, and is targeted by potent, selective small-molecule inhibitors currently in clinical trials for tumors harboring MTAP deletions.

免疫印迹
- WB result of PRMT5 Recombinant Rabbit mAb
  Primary antibody: PRMT5 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HEK-293 whole cell lysate 20 µg
  Lane 3: HepG2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 72 kDa
  Observed MW: 70 kDa
- WB result of PRMT5 Recombinant Rabbit mAb
  Primary antibody: PRMT5 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C2C12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 72 kDa
  Observed MW: 70 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human stomach. Anti-PRMT5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostate. Anti-PRMT5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-PRMT5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-PRMT5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PRMT5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti-PRMT5 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).