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RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb (S-3420)

DNA-directed RNA polymerase II subunit RPB1,RNA polymerase II subunit B1,3'-5' exoribonuclease,DNA-directed RNA polymerase II subunit A,DNA-directed RNA polymerase III largest subunit,RNA-directed RNA polymerase II subunit RPB1,POLR2,POLR2A

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6306

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Mouse
抗原名称

RNA polymerase II CTD repeat YSPTSPS (phospho S5)
分子别名

DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; 3'-5' exoribonuclease; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1; POLR2; POLR2A
细胞定位

Cytoplasm, Nucleus
Accession

P24928
克隆号

S-3420
抗体类型

Mouse mAb
抗体同种型

IgG1
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, 293T, NIH/3T3
纯化方式

Protein G
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:2000 Hu, Ms, Rt
ICC 1:500 Hu, Ms
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
WB	1:1000-1:2000	Hu, Ms, Rt
ICC	1:500	Hu, Ms
ChIP	1:20-1:50	Hu

背景介绍

RNA polymerase II CTD repeat YSPTSPS (phospho S5) is a crucial component of the RNA polymerase II complex, specifically referring to the phosphorylated serine 5 residue within the heptapeptide repeat sequence YSPTSPS in the CTD (C-terminal domain) of the largest subunit of RNA polymerase II. This phosphorylation of serine 5 plays a significant role in the transcription process, particularly during the initiation and early elongation phases. It helps in the recruitment of transcription factors and other regulatory proteins, facilitating the assembly of the pre-initiation complex and enabling efficient transcription initiation. Additionally, the phosphorylation status of S5 can influence the interaction of RNA polymerase II with other components of the transcription machinery, thereby regulating gene expression and ensuring proper processing of nascent RNA transcripts.

免疫印迹
- WB result of RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb
  at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: 293T whole cell lysate 20 µg
  Lane 3: HeLa treated with phosphatase whole cell lysate 20 µg
  Lane 4: 293T treated with phosphatase whole cell lysate 20 µg
  Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 218 kDa
  Observed MW: 280 kDa
- WB result of RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb
  at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 treated with phosphatase whole cell lysate 20 µg
  Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 218 kDa
  Observed MW: 280 kDa
- WB result of RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb
  at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: PC-12 treated with phosphatase whole cell lysate 20 µg
  Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 218 kDa
  Observed MW: 280 kDa
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb (S-3420) and beads only at 1:50 for immunoprecipitation.
  Post -immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR (%input: immunoprecipitated DNA/input DNA)
  showed the enrichment of RPL30, GAPDH, MYOD1,
  AFM, SAT-α and SAT-2 in RNA polymerase II CTD repeat
  YSPTSPS (phospho S5) Recombinant Mouse mAb (S-3420)-
  immunoprecipitated sample.