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Rabbit抗原名称
AACT分子别名
Alpha-1-antichymotrypsin; ACT; Cell growth-inhibiting gene 24/25 protein; Serpin A3; SERPINA3免疫原
Recombinant Protein细胞定位
SecretedAccession
P01011克隆号
S-897-109抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
human serum, human plasma纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:10000 Hu IHC-P 1:1000 Hu
Alpha-1-antichymotrypsin (AACT), also known as SERPINA3, is a serine protease inhibitor primarily synthesized in the liver and secreted into the blood. It is an acute-phase protein involved in the inflammatory response and has a molecular weight of approximately 47.651 kDa. AACT plays a crucial role in inhibiting proteases such as cathepsin G and chymase, thereby protecting cells and tissues from proteolytic damage. Elevated levels of AACT have been observed in various diseases, including Alzheimer's disease, heart failure, and multiple cancers. Its expression and glycosylation patterns can serve as potential biomarkers for disease diagnosis and prognosis.
免疫印迹
WB result of AACT Recombinant Rabbit mAb
Primary antibody: AACT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: human serum lysate 5 µg
Lane 2: human plasma lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 60-70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-AACT antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-AACT antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-AACT antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-AACT antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-AACT antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-AACT antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of AACT in tumor tissue.
Expression of AACT in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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