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active YAP1 Recombinant Rabbit mAb (S-3261)

Transcriptional coactivator YAP1,Yes-associated protein 1,Protein yorkie homolog,Yes-associated protein YAP65 homolog,YAP65

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6302
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产品介绍

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 分子别名

    Transcriptional coactivator YAP1; Yes-associated protein 1; Protein yorkie homolog; Yes-associated protein YAP65 homolog; YAP65

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    P46937

  • 克隆号

    S-3261

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HUVEC

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
    IHC-P 1:500 Hu, Ms, Rt
    ICC 1:500 Hu
背景介绍

  • YAP1, also known as YAP (Yes-Associated Protein) or WWTR1 (WW Domain Containing Transcription Regulator 1), is a transcriptional co-activator that plays a pivotal role in the Hippo signaling pathway. When YAP1 is in an active state (commonly referred to as active YAP1), it enters the nucleus and binds to transcription factors called TEADs (TEA Domain Family Members). This YAP-TEAD complex then promotes the expression of a series of genes associated with cell proliferation, survival, and migration. However, when the Hippo signaling pathway is activated, the activity of YAP1 is suppressed. This often involves the phosphorylation of YAP1, which leads to its binding with 14-3-3 proteins and retention in the cytoplasm, preventing it from entering the nucleus to activate transcription. Abnormal expression or dysfunction of active YAP1 has been linked to various diseases, including cancer, heart disease, and neurological disorders. Therefore, studying the regulatory mechanisms of YAP1 activity and its role in diseases is crucial for developing new therapeutic strategies.

  • 免疫印迹

    • WB result of active YAP1 Recombinant Rabbit mAb
      Primary antibody: active YAP1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa treated with 100ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
      Lane 2: HeLa treated with 100ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg, then
      the membrane was treated with Alkaline Phosphatase for l hour
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 54 kDa
      Observed MW: 85 kDa

    • WB result of active YAP1 Recombinant Rabbit mAb
      Primary antibody: active YAP1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 treated with 100ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with 100ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg, then the membrane was treated with Alkaline Phosphatase for l hour
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 75 kDa
      Observed MW: 80 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-active YAP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human testis. Anti-active YAP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-active YAP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-active YAP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse testis. Anti-active YAP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat testis. Anti-active YAP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HUVEC cells. Anti-active YAP1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of active YAP1 in tumor tissue.

    • Expression of active YAP1 in human tissue.

    • Expression of active YAP1 in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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