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Rabbit抗原名称
ADAM8分子别名
Disintegrin and metalloproteinase domain-containing protein 8; Cell surface antigen MS2; CD156a; MS2免疫原
Recombinant Protein细胞定位
MembraneAccession
P78325克隆号
S-2546-23抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
MDA-MB-231, Capan-1, BxPC-3纯化方式
Protein G浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
ADAM8 (a disintegrin and metalloproteinase domain 8), also designated CD156a, is a type-I transmembrane metalloproteinase encoded at 10q26.3 that belongs to the ADAM family of cell-surface sheddases; its modular architecture comprises a pro-domain, catalytic metalloprotease, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic tail domains, endowing it with the dual capacity to cleave extracellular-matrix proteins such as fibronectin and collagen while simultaneously regulating cell–cell and cell–matrix interactions through its disintegrin motif. Physiologically, ADAM8 is expressed at highest levels in immune and neuronal tissues, where it modulates leukocyte transmigration, cytokine and chemokine shedding (e.g., TNF-α, CX3CL1), and neuronal adhesion during development and repair; pathologically, its marked up-regulation in cancers promotes epithelial-to-mesenchymal transition, angiogenesis, and metastasis by attenuating intercellular adhesion and degrading matrix barriers, and in allergic asthma it orchestrates airway remodeling and hyper-responsiveness, rendering ADAM8 a prognostic biomarker and an attractive therapeutic target for anti-metastatic and anti-inflammatory interventions .
免疫印迹
WB result of ADAM8 Recombinant Rabbit mAb
Primary antibody: ADAM8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: MDA-MB-231 whole cell lysate 20 µg
Lane 3: Capan-1 whole cell lysate 20 µg
Lane 4: BxPC-3 whole cell lysate 20 µg
Negative control: PANC-1 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 89 kDa
Observed MW: 80-120 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,我们对一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定







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