热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 抗体 其他一抗

所有图片（1/15）

Argonaute 2 Recombinant Rabbit mAb (S-2620-13)

Protein argonaute-2,mAgo2,Argonaute RISC catalytic component 2,Eukaryotic translation initiation factor 2C 2 (eIF-2C 2,eIF2C 2),Piwi/argonaute family protein meIF2C2,Protein slicer,Eif2c2,Kiaa4215,Ago2

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6300
规格
数量
收藏 分享
产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Argonaute 2

  • 分子别名

    Protein argonaute-2; mAgo2; Argonaute RISC catalytic component 2; Eukaryotic translation initiation factor 2C 2 (eIF-2C 2; eIF2C 2); Piwi/argonaute family protein meIF2C2; Protein slicer; Eif2c2; Kiaa4215; Ago2

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    Q8CJG0

  • 克隆号

    S-2620-13

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, Jurkat, MCF7, HepG2, U-87 MG, HCT 116, RAW264.7, mouse kidney, C6

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
    IHC-P 1:250 Hu, Ms, Rt
    ICC 1:100 Hu
背景介绍

  • Argonaute 2 (AGO2) is the only catalytically active member of the four mammalian Argonaute proteins, acting as the core effector of RNA interference by loading ~22-nt microRNAs or siRNAs to form the RNA-induced silencing complex (RISC), in which its PIWI endonuclease domain can cleave perfectly complementary mRNAs while mismatched targets are repressed translationally via deadenylation and P-body sequestration; uniquely indispensable for vertebrate development, AGO2 also participates in transcriptional gene silencing, antiviral defense, and stress-granule dynamics, and its expression and post-translational modifications are frequently dysregulated in cancers .

  • 免疫印迹

    • WB result of Argonaute 2 Recombinant Rabbit mAb
      Primary antibody: Argonaute 2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg
      Lane 3: MCF7 whole cell lysate 20 µg
      Lane 4: HepG2 whole cell lysate 20 µg
      Lane 5: U-87 MG whole cell lysate 20 µg
      Lane 6: HCT 116 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 97 kDa
      Observed MW: 97 kDa

    • WB result of Argonaute 2 Recombinant Rabbit mAb
      Primary antibody: Argonaute 2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: RAW264.7 whole cell lysate 20 µg
      Lane 2: mouse kidney lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 97 kDa
      Observed MW: 97 kDa

    • WB result of Argonaute 2 Recombinant Rabbit mAb
      Primary antibody: Argonaute 2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 97 kDa
      Observed MW: 97 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Argonaute 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Argonaute 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human placenta. Anti-Argonaute 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Argonaute 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Argonaute 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Argonaute 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-Argonaute 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Argonaute 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- Argonaute 2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Argonaute 2 in tumor tissue.

    • Expression of Argonaute 2 in human tissue.

    • Expression of Argonaute 2 in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

评论(0)