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ATG14 Recombinant Rabbit mAb (S-2371-15)

Beclin 1-associated autophagy-related key regulator,Barkor,Autophagy-related protein 14-like protein (Atg14L),ATG14L,KIAA0831

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6299
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    ATG14

  • 分子别名

    Beclin 1-associated autophagy-related key regulator; Barkor; Autophagy-related protein 14-like protein (Atg14L); ATG14L; KIAA0831

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Endoplasmic reticulum membrane, Cytoplasm

  • Accession

    Q6ZNE5

  • 克隆号

    S-2371-15

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt, Mk

  • 阳性样本

    HeLa, HCT 116, SK-OV-3, HepG2, NIH/3T3, Neuro-2a, mouse brain, mouse testis, PC-12, rat brain, rat testis, COS-7

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt, Mk
背景介绍

  • ATG14, also known as Barkor (Beclin 1-associated autophagy-related key regulator), is a 492-amino-acid protein essential for autophagy initiation in eukaryotes. It assembles with Beclin 1, VPS15, and the class III phosphatidylinositol 3-kinase VPS34 to form the PI3K complex I that nucleates isolation membranes and omegasomes, thereby triggering autophagosome biogenesis. ATG14 recognizes membrane curvature via its N-terminal cysteine-rich domain, which binds phosphatidylinositol 3-phosphate to tether pre-autophagosomal membranes, and subsequently promotes STX17-SNAP29 t-SNARE complex assembly to drive autophagosome–lysosome fusion [^3][^8]. Beyond canonical autophagy, ATG14 safeguards mitochondrial integrity and restrains gasdermin-mediated pyroptosis, enabling physiological processes such as hepatic lipid homeostasis and early-pregnancy embryo transport.

  • 免疫印迹

    • WB result of ATG14 Recombinant Rabbit mAb
      Primary antibody: ATG14 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: HCT 116 whole cell lysate 20 µg
      Lane 3: SK-OV-3 whole cell lysate 20 µg
      Lane 4: HepG2 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 55 kDa
      Observed MW: 58, 68 kDa

    • WB result of ATG14 Recombinant Rabbit mAb
      Primary antibody: ATG14 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: Neuro-2a whole cell lysate 20 µg
      Lane 3: mouse brain lysate 20 µg
      Lane 4: mouse testis lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 55 kDa
      Observed MW: 58, 68 kDa

    • WB result of ATG14 Recombinant Rabbit mAb
      Primary antibody: ATG14 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Lane 2: rat brain lysate 20 µg
      Lane 3: rat testis lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 55 kDa
      Observed MW: 58, 68 kDa

    • WB result of ATG14 Recombinant Rabbit mAb
      Primary antibody: ATG14 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: COS-7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 55 kDa
      Observed MW: 55, 70 kDa

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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