c-IAP2 Recombinant Rabbit mAb (S-3395)
Baculoviral IAP repeat-containing protein 3,Apoptosis inhibitor 2 (API2),Cellular inhibitor of apoptosis 2 (C-IAP2),IAP homolog C,Inhibitor of apoptosis protein 1 (hIAP-1,hIAP1),RING finger protein 49,API2,MIHC,RNF49,BIRC3
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宿主来源
Rabbit抗原名称
c-IAP2分子别名
Baculoviral IAP repeat-containing protein 3; Apoptosis inhibitor 2 (API2); Cellular inhibitor of apoptosis 2 (C-IAP2); IAP homolog C; Inhibitor of apoptosis protein 1 (hIAP-1; hIAP1); RING finger protein 49; API2; MIHC; RNF49; BIRC3细胞定位
Cytoplasm, NucleusAccession
Q13489克隆号
S-3395抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Raji, Ramos, Daudi纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu
Cellular inhibitor of apoptosis protein 2 (c-IAP2, gene BIRC3) is a 618-residue member of the IAP family containing three N-terminal baculoviral IAP repeat (BIR) domains that bind caspases, a central caspase-recruitment domain (CARD) that auto-inhibits its own ubiquitin-ligase activity, and a C-terminal RING domain that endows potent E3 ubiquitin-ligase activity; through this enzymatic function c-IAP2 polyubiquitinates receptor-interacting protein 1 (RIP1) and NF-κB-inducing kinase (NIK), thereby driving canonical NF-κB prosurvival signaling while suppressing non-canonical NF-κB activation, controls TNF-α-mediated apoptosis, regulates innate immunity downstream of Toll-like and NOD-like receptors, and is frequently amplified or over-expressed in multiple cancers where its anti-apoptotic activity contributes to chemoresistance, making it a prime target for small-molecule Smac mimetics that induce its autoubiquitination and proteasomal degradation.
免疫印迹
WB result of cIAP2 Recombinant Rabbit mAb
Primary antibody: cIAP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: Ramos whole cell lysate 20 µg
Lane 3: Daudi whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti- c-IAP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti- c-IAP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti- c-IAP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of c-IAP2 in tumor tissue.
Expression of c-IAP2 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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