分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
E2F-1分子别名
Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1 (RBAP-1); Retinoblastoma-binding protein 3 (RBBP-3); pRB-binding protein E2F-1; RBBP3; E2F1免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q01094克隆号
S-2580-78抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HEK-293, HeLa纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu ChIP 1:20-1:50 Hu
E2F-1 is a 437-amino-acid transcription factor that sits at the apex of cell-cycle control, binding as a heterodimer with DP-1 to the consensus TTTCCCGC element in promoters of genes encoding S-phase regulators such as cyclin E, cyclin A and DNA polymerase-α; its activity is restrained in G0/G1 by direct association with the tumor-suppressor Rb, and upon mitogenic phosphorylation of Rb by cyclin-D-dependent kinases E2F-1 is liberated to activate transcription, while DNA damage triggers ATM/ATR-mediated phosphorylation and stabilization of E2F-1, converting it into a potent inducer of p53-independent apoptosis via transcriptional up-regulation of p73, Apaf-1 and caspases; accordingly, E2F-1 acts as both a canonical driver of proliferation and, under stress, a guardian against oncogenic transformation, and its gene is amplified or overexpressed in several human cancers yet germ-line polymorphisms that reduce E2F-1 levels are linked to increased lymphoma risk, illustrating the delicate balance of E2F-1’s dualistic role in life and death decisions of the cell.
免疫印迹
WB result of E2F-1 Recombinant Rabbit mAb
Primary antibody: E2F-1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 62 kDa
This blot was developed with high sensitivity substrate
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used E2F-1 Recombinant Rabbit mAb (S-2580-78) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of TIMELESS, DHFR and SAT-α in E2F-1 Recombinant
Rabbit mAb (S-2580-78)-immunoprecipitated sample.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)