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FOXM1 Recombinant Rabbit mAb (S-2529-26)

Forkhead box protein M1,Forkhead-related protein FKHL16,Hepatocyte nuclear factor 3 forkhead homolog 11 (HFH-11,HNF-3/fork-head homolog 11),M-phase phosphoprotein 2,MPM-2 reactive phosphoprotein 2,Transcription factor Trident,Winged-helix factor from INS-1 cells,FKHL16,HFH11,MPP2,WIN

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6296

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

FOXM1
分子别名

Forkhead box protein M1; Forkhead-related protein FKHL16; Hepatocyte nuclear factor 3 forkhead homolog 11 (HFH-11; HNF-3/fork-head homolog 11); M-phase phosphoprotein 2; MPM-2 reactive phosphoprotein 2; Transcription factor Trident; Winged-helix factor from INS-1 cells; FKHL16; HFH11; MPP2; WIN
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

Q08050
克隆号

S-2529-26
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

HT-29, A431, HeLa
纯化方式

Protein A
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
IHC-P 1:200 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu
IHC-P	1:200	Hu, Ms, Rt

背景介绍

FOXM1 is a transcription factor of the Forkhead box family that orchestrates cell-cycle progression by directly activating genes required for G1/S and G2/M transitions, DNA replication, and mitosis; its expression is normally low in quiescent cells but rises sharply from late G1 through M phase, and it is frequently over-expressed in human cancers where it promotes proliferation, angiogenesis, metastasis, and chemo-radiotherapy resistance through pathways including PI3K/AKT, Wnt/β-catenin, and p53; post-translational modifications such as phosphorylation by CDK, PLK1 and AKT, together with ubiquitin-mediated degradation, tightly regulate FOXM1 activity, making it both a key biomarker for poor prognosis and an attractive therapeutic target in oncology.

免疫印迹
- WB result of FOXM1 Recombinant Rabbit mAb
  Primary antibody: FOXM1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HT-29 whole cell lysate 20 µg
  Lane 2: A431 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 84 kDa
  Observed MW: 110 kDa
  This blot was developed with high sensitivity substrate
- WB result of FOXM1 Recombinant Rabbit mAb
  Primary antibody: FOXM1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 100 ng/ml Nocodazole for 17 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 84 kDa
  Observed MW: 110 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human testis. Anti-FOXM1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-FOXM1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-FOXM1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-FOXM1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-FOXM1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-FOXM1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti-FOXM1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-FOXM1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of FOXM1 in tumor tissue.
- Expression of FOXM1 in human tissue.
- Expression of FOXM1 in mouse & rat tissue.