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KIF5B+KIF5C Recombinant Rabbit mAb (S-2452-15)

Kinesin-1 heavy chain,Conventional kinesin heavy chain,Ubiquitous kinesin heavy chain (UKHC),KNS,KNS1,Kinesin heavy chain isoform 5C,Kinesin heavy chain neuron-specific 2,Kinesin-1,KIF5C,KIAA0531,NKHC2

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6294
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    KIF5B

  • 分子别名

    Kinesin-1 heavy chain; Conventional kinesin heavy chain; Ubiquitous kinesin heavy chain (UKHC); KNS; KNS1; Kinesin heavy chain isoform 5C; Kinesin heavy chain neuron-specific 2; Kinesin-1; KIF5C; KIAA0531; NKHC2

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Cytoskeleton

  • Accession

    P33176, O60282

  • 克隆号

    S-2452-15

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, Jurkat, MCF7, HCT 116, HepG2, Neuro-2a, C2C12, mouse brain, rat brain

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ICFCM

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:2000 Hu, Ms, Rt
    IHC-P 1:250 Hu, Ms, Rt
    ICC 1:100 Hu
    ICFCM 1:50 Hu
背景介绍

  • KIF5B and KIF5C are two major members of the Kinesin-1 family of motor proteins, encoded by distinct genes. They share a similar core structure, each containing a conserved N-terminal motor domain (which binds to microtubules and hydrolyzes ATP to generate motive force), a neck linker region, a coiled-coil stalk region (mediating dimerization), and a C-terminal tail domain (responsible for binding to various cargoes and regulation). However, they exhibit key differences in tissue distribution and function. KIF5B is widely expressed in nearly all cell types and is responsible for fundamental "housekeeping" transport processes, such as the movement of organelles like mitochondria, endoplasmic reticulum, and endosomes along microtubules. In contrast, KIF5C is predominantly and highly expressed in neurons, making it a neuron-specific kinesin critical for neuronal development and function. It is essential for the anterograde transport of specific cargoes, including presynaptic components, vesicles, and mRNAs, from the neuronal cell body along the axon to distal synapses. This transport is indispensable for maintaining neuronal polarity and synaptic plasticity. The differences in their tail domains determine their ability to recognize and bind distinct cargo adaptor proteins, enabling fine-tuned functional specialization in intracellular transport.

  • 免疫印迹

    • WB result of KIF5B+KIF5C Recombinant Rabbit mAb
      Primary antibody: KIF5B Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg
      Lane 3: MCF7 whole cell lysate 20 µg
      Lane 4: HCT 116 whole cell lysate 20 µg
      Lane 5: HepG2 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 110 kDa
      Observed MW: 115 kDa

    • WB result of KIF5B+KIF5C Recombinant Rabbit mAb
      Primary antibody: KIF5B+KIF5C Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Neuro-2a whole cell lysate 20 µg
      Lane 2: C2C12 whole cell lysate 20 µg
      Lane 3: mouse brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 110 kDa
      Observed MW: 115 kDa

    • WB result of KIF5B+KIF5C Recombinant Rabbit mAb
      Primary antibody: KIF5B+KIF5C Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 110 kDa
      Observed MW: 120 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labelling KIF5B+KIF5C antibody at 1/50 dilution (1 μg)/ (red) compared with a Rabbit monoclonal IgG (black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-KIF5B+KIF5C antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human testis. Anti-KIF5B+KIF5C antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-KIF5B+KIF5C antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human pancreatic. Anti-KIF5B+KIF5C antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-KIF5B+KIF5C antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-KIF5B+KIF5C antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat colon. Anti-KIF5B+KIF5C antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-KIF5B+KIF5C antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of KIF5B in tumor tissue.

    • Expression of KIF5B in human tissue.

    • Expression of KIF5B in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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