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Phospho- Zap-70 (Tyr319)/Syk (Tyr352) Recombinant Rabbit mAb (S-1252-12)

Tyrosine-protein kinase ZAP-70,70 kDa zeta-chain associated protein,Syk-related tyrosine kinase,SRK,ZAP70,Tyrosine-protein kinase SYK,Spleen tyrosine kinase,p72-Syk,SYK

Datasheet COA

价格￥600.00 （现货： 2-5个工作日）

货号 S0B6293

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho- Zap-70 (Tyr319)/Syk (Tyr352)
分子别名

Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase; SRK; ZAP70; Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk; SYK
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Cytoskeleton, Cell membrane, Synapse
Accession

P43403, P43405
克隆号

S-1252-12
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000-1:2000 Hu, Rt
IHC-P 1:500 Hu, Ms, Rt
ICC 1:500 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000-1:2000	Hu, Rt
IHC-P	1:500	Hu, Ms, Rt
ICC	1:500	Hu

背景介绍

Phospho-Zap-70 (Tyr319)/Syk (Tyr352) is the dually phosphorylated, catalytically active form of the tandem SH2-bearing Syk family tyrosine kinases Zap-70 and Syk, wherein phosphorylation of Tyr319 in Zap-70 (or the homologous Tyr352 in Syk) within the interdomain B linker induces an open conformation that relieves autoinhibition, promotes recruitment to doubly phosphorylated ITAM motifs of the T-cell receptor (Zap-70) or B-cell receptor (Syk) signaling complexes, and creates docking sites for downstream adaptors such as LAT and BLNK, thereby propagating calcium flux, MAPK activation, and transcriptional programs essential for lymphocyte development, activation, and effector function.

免疫印迹
- WB result of Phospho- Zap-70 (Tyr319)/Syk (Tyr352) Recombinant Rabbit mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho- Zap-70 (Tyr319)/Syk (Tyr352) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated Ramos whole cell lysate 20 µg
  Lane 2: Serum-starved Ramos treated with 10 mM pervanadate for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 70 kDa
  Observed MW: 65-75 kDa
- WB result of Phospho- Zap-70 (Tyr319)/Syk (Tyr352) Recombinant Rabbit mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho- Zap-70 (Tyr319)/Syk (Tyr352) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated C6 whole cell lysate 20 µg
  Lane 2: C6 treated with 10 mM pervanadate for 20 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 70 kDa
  Observed MW: 60-80 kDa
斑点杂交
- Dot blot result of Phospho-Zap-70(Tyr319)/Syk(Tyr352) Recombinant Rabbit mAb
  Lane 1: Zap-70 (Tyr319) phospho peptide 1
  Lane 2: Zap-70 (Tyr319) phospho peptide 2
  Lane 3: Zap-70 unmodified peptide
  Primary antibody: Phospho-Zap-70(Tyr319)/Syk(Tyr352) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil and negative staining in human tonsil treated with phosphatase. Anti- Phospho- Zap-70 (Tyr319)/Syk (Tyr352) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse spleen and negative staining in mouse spleen treated with phosphatase. Anti- Phospho- Zap-70 (Tyr319)/Syk (Tyr352) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat spleen and negative staining in rat spleen treated with phosphatase. Anti- Phospho- Zap-70 (Tyr319)/Syk (Tyr352) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC analysis of Ramos cells starve overnight then treated with pervanadate (10mM,30min) (top panel) and untreated Ramos cells (below panel). Anti- Phospho- Zap-70 (Tyr319) Syk (Tyr352) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).