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Rabbit抗原名称
PKA C-α分子别名
cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PKACA; PRKACA免疫原
Synthetic Peptide细胞定位
Cytoplasm, Nucleus, Cell membraneAccession
P17612克隆号
S-2564-5抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa, 293T, MCF7, NIH/3T3, mouse brain, mouse kidney, C6, rat brain, rat kidney预测反应种属
(反应种属缩写表)Bv, Dg, Hm, Sh纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms, Rt, Mk IHC-P 1:1000 Hu, Ms, Rt
PKA C-α protein, fully known as cAMP-dependent protein kinase A catalytic subunit alpha, is a crucial signal transduction molecule within cells, often regarded as one of the fundamental "molecular switches" of life. As the core catalytic component of the PKA holoenzyme, it exists in an inactive state bound to regulatory subunits. When the levels of the second messenger cAMP rise, cAMP binds to the regulatory subunits, causing their dissociation from the catalytic subunit and thereby activating PKA C-α. Once activated, PKA C-α exerts its function by transferring a phosphate group from ATP to specific serine or threonine residues on its target substrate proteins, a process known as reversible phosphorylation. This mechanism precisely regulates a vast array of core physiological functions, including glycogen metabolism, lipid synthesis, gene transcription, cell proliferation and differentiation, learning and memory, and heart rhythm. Dysregulation of its activity or expression is closely associated with various human diseases, such as cancer, heart disease, metabolic disorders, and brain dysfunction.
免疫印迹
WB result of PKA C-α Recombinant Rabbit mAb
Primary antibody: PKA C-α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 39 kDaWB result of PKA C-α Recombinant Rabbit mAb
Primary antibody: PKA C-α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 39 kDaWB result of PKA C-α Recombinant Rabbit mAb
Primary antibody: PKA C-α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 39 kDaWB result of PKA C-α Recombinant Rabbit mAb
Primary antibody: PKA C-α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 39 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti- PKA C-α antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti- PKA C-α antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- PKA C-α antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti- PKA C-α antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- PKA C-α antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- PKA C-α antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of PKA C-α in tumor tissue.
Expression of PKA C-α in human tissue.
Expression of PKA C-α in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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