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Rabbit抗原名称
RIPK1分子别名
Receptor-interacting serine/threonine-protein kinase 1; Cell death protein RIP; Receptor-interacting protein 1 (RIP-1); RIP; RIP1; RIPK1免疫原
Recombinant Protein细胞定位
Cytoplasm, Cell membraneAccession
Q13546抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HT-29, HL-60, Raji, HeLa, NIH/3T3, mouse kidney, mouse heart, C6, rat kidney纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu, Ms, Rt IHC-P 1:200 Hu, Ms, Rt
RIPK1 (Receptor-Interacting Serine/Threonine-Protein Kinase 1) is a critical decision-maker of cell fate, a multifunctional protein that plays a central role at the crossroads of cell survival, inflammation, and death signaling pathways. Initially identified as a key component of the tumor necrosis factor receptor 1 (TNFR1) signaling complex, it performs dual roles through its kinase activity and scaffolding function: on one hand, it promotes the transcription of pro-survival genes and inflammatory responses by recruiting and activating downstream signaling molecules (such as the NF-κB and MAPK pathways); on the other hand, under specific conditions, RIPK1 can become a powerful driver of cell death, capable of activating caspase-8-mediated apoptosis or participating in the formation of a phosphorylated MLKL-mediated necroptosis complex. Consequently, the activity of RIPK1 is tightly regulated by various post-translational modifications, including ubiquitination, phosphorylation, and caspase-mediated cleavage. Dysregulation of its function is closely associated with numerous pathological processes such as sepsis, neurodegenerative diseases (like Alzheimer's disease and amyotrophic lateral sclerosis), autoimmune disorders, and ischemic injury.
免疫印迹
WB result of RIPK1 Rabbit pAb
Primary antibody: RIPK1 Rabbit pAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Lane 2: HL-60 whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 76 kDa
Observed MW: 47, 70 kDa
This blot was developed with high sensitivity substrateWB result of RIPK1 Rabbit pAb
Primary antibody: RIPK1 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Lane 3: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 76 kDa
Observed MW: 47, 70 kDa
This blot was developed with high sensitivity substrateWB result of RIPK1 Rabbit pAb
Primary antibody: RIPK1 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 76 kDa
Observed MW: 47, 70 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-RIPK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-RIPK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-RIPK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-RIPK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-RIPK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-RIPK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of PIPK1 in tumor tissue.
Expression of PIPK1 in human tissue.
Expression of PIPK1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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