TNF alpha Recombinant Rabbit mAb (S-2506-69)

Tumor necrosis factor,Cachectin,TNF-alpha,Tumor necrosis factor ligand superfamily member 2 (TNF-a),TNFA,TNFSF2,TNF

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6287

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

TNF alpha
分子别名

Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2 (TNF-a); TNFA; TNFSF2; TNF
免疫原

Synthetic Peptide
细胞定位

Secreted, Cell membrane
Accession

P01375
克隆号

S-2506-69
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
RRID

AB_3751393
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:2000 Hu, Ms

应用	稀释度	推荐种属
WB	1:1000-1:2000	Hu, Ms

背景介绍

Tumor necrosis factor alpha (TNF-α) is a pleiotropic, 17-kDa homotrimeric cytokine synthesized chiefly by activated macrophages but also by T cells, NK cells, fibroblasts, and adipocytes; it signals through two distinct receptors, TNFR1 and TNFR2, to orchestrate a vast array of biological processes including activation of NF-κB and MAPK pathways that drive pro-inflammatory gene expression, induction of fever, chemokine secretion for leukocyte recruitment, enhancement of cytotoxic CD8+ T cell responses, and promotion of apoptosis or necroptosis via caspase-8 or RIPK1/RIPK3 signaling, while also mediating cachexia and insulin resistance in chronic inflammation, and its dysregulated overproduction is a central driver of rheumatoid arthritis, inflammatory bowel disease, psoriasis, sepsis, and neurodegeneration, making it a prime therapeutic target for monoclonal antibodies such as infliximab, adalimumab, and certolizumab pegol.

免疫印迹
- WB result of TNF alpha Recombinant Rabbit mAb
  Primary antibody: TNF alpha Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated THP-1 whole cell lysate 20 µg
  Lane 2: THP-1 treated with 80 nM TPA and 1 ug/ml LPS overnight, then add 500 ng/ml BFA for 6 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 25 kDa
  Observed MW: 23 kDa
- WB result of TNF alpha Recombinant Rabbit mAb
  Primary antibody: TNF alpha Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated RAW 264.7 whole cell lysate 20 µg
  Lane 2: RAW 264.7 treated with 100 ng LPS for 6 hrs, stimulation with 300 ng/ml BFA at the last 3 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 25 kDa
  Observed MW: 23, 27 kDa
免疫细胞化学
- ICC analysis of THP-1 cells treated with TPA (80nM, overnight) and LPS (1ug/ml, overnight) and BFA (500ng/ml, last 6 hrs) (top panel) and untreated THP-1 cells (below panel). Anti- TNF alpha antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).