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Rabbit抗原名称
ADRB2分子别名
Beta-2 adrenergic receptor; Beta-2 adrenoreceptor (Beta-2 adrenoceptor); ADRB2R; B2AR免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P07550克隆号
S-2418-144抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
mouse heart, rat heart纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:1000 Hu, Ms, Rt IF 1:100 Ms
The ADRB2 (β2-adrenergic receptor) is a 413-amino-acid G-protein-coupled receptor embedded in the plasma membrane of airway, vascular, and adipose tissues, where it binds epinephrine and norepinephrine to trigger intracellular signaling cascades via Gs-mediated adenylyl cyclase activation, elevating cAMP and PKA to relax bronchial and vascular smooth muscle, modulate lipolysis, and influence cardiac output; encoded on chromosome 5q31–32, ADRB2 is highly polymorphic—most notably the Arg16Gly and Gln27Glu variants that alter down-regulation and desensitization—making it a pharmacogenomic target for β2-agonists in asthma and COPD therapy, and its structure has been captured in both active and inactive conformations by X-ray crystallography, revealing the molecular basis of agonist efficacy, inverse agonism, and biased signaling exploited in modern drug design.
免疫印迹
WB result of ADRB2 Recombinant Rabbit mAb
Primary antibody: ADRB2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 46 kDaWB result of ADRB2 Recombinant Rabbit mAb
Primary antibody: ADRB2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 46 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human stomach. Anti-ADRB2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-ADRB2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-ADRB2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-ADRB2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-ADRB2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse heart. Anti- ADRB2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of ADRB2 in tumor tissue.
Expression of ADRB2 in human tissue.
Expression of ADRB2 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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