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BACE1 Recombinant Rabbit mAb (S-2514-34)

Beta-secretase 1,Aspartyl protease 2 (ASP2,Asp 2),Beta-site amyloid precursor protein cleaving enzyme 1 (Beta-site APP cleaving enzyme 1),Memapsin-2,Membrane-associated aspartic protease 2,Bace1,Bace

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6280

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

BACE1
分子别名

Beta-secretase 1; Aspartyl protease 2 (ASP2; Asp 2); Beta-site amyloid precursor protein cleaving enzyme 1 (Beta-site APP cleaving enzyme 1); Memapsin-2; Membrane-associated aspartic protease 2; Bace1; Bace
免疫原

Synthetic Peptide
细胞定位

Lysosome, Cell membrane, Endosome, Endoplasmic reticulum
Accession

P56818
克隆号

S-2514-34
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

Neuro-2a, mouse brain, PC-12, rat brain
预测反应种属
(反应种属缩写表)

Bv, GP
纯化方式

Protein A
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:500-1:1000 Hu, Ms, Rt
IHC-P 1:200-1:1000 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:500-1:1000	Hu, Ms, Rt
IHC-P	1:200-1:1000	Hu, Ms, Rt

背景介绍

BACE1 (β-site amyloid precursor protein cleaving enzyme 1), a 501-residue type-I transmembrane aspartyl protease encoded by the BACE1 gene on chromosome 11q23.3, is the rate-limiting enzyme that initiates amyloid-β (Aβ) generation by cleaving APP at the β-secretase site within acidic endosomes; its activity is highest in neurons, modulated by post-translational glycosylation, phosphorylation and trafficking to lipid rafts, and is tightly regulated by spatial segregation, endogenous inhibitors like BRI2, and a short isoform BACE1-457 that lacks the catalytic domain; genetic deletion or pharmacological inhibition in rodents reduces Aβ pathology and reverses synaptic deficits with minimal toxicity, making BACE1 a prime yet challenging therapeutic target for Alzheimer’s disease, although clinical trials have been complicated by mechanism-based side effects including retinal and cognitive abnormalities due to cleavage of additional substrates such as neuregulin-1, sodium channel β-subunits and seizure-related proteins.

免疫印迹
- WB result of BACE1 Recombinant Rabbit mAb
  Primary antibody: BACE1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Raji whole cell lysate 20 µg
  Lane 2: SH-SY5Y whole cell lysate 20 µg
  Negative control: Raji whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 70 kDa
  This blot was developed with high sensitivity substrate
- WB result of BACE1 Recombinant Rabbit mAb
  Primary antibody: BACE1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: RAW264.7 whole cell lysate 20 µg
  Lane 2: Neuro-2a whole cell lysate 20 µg
  Lane 3: mouse brain lysate 20 µg
  Negative control: RAW264.7 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 70 kDa
  This blot was developed with high sensitivity substrate
- WB result of BACE1 Recombinant Rabbit mAb
  Primary antibody: BACE1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 65, 75 kDa
  This blot was developed with high sensitivity substrate
免疫组化
- IHC shows positive staining in paraffin-embedded human glioblastoma. Anti-BACE1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse hippocampus. Anti-BACE1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-BACE1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat hippocampus. Anti-BACE1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded rat liver. Anti-BACE1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of BACE1 in tumor tissue.
- Expression of BACE1 in human tissue.
- Expression of BACE1 in mouse & rat tissue.