BAFF Recombinant Rabbit mAb (S-2175-109)
Tumor necrosis factor ligand superfamily member 13B,B lymphocyte stimulator (BLyS),B-cell-activating factor,BAFF,Dendritic cell-derived TNF-like molecule,TNF- and APOL-related leukocyte expressed ligand 1 (TALL-1),CD257,BLYS,TALL1,TNFSF20,ZTNF4,TNFSF13B
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宿主来源
Rabbit抗原名称
BAFF分子别名
Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator (BLyS); B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1 (TALL-1); CD257; BLYS; TALL1; TNFSF20; ZTNF4; TNFSF13B免疫原
Recombinant Protein细胞定位
Cell membraneAccession
Q9Y275克隆号
S-2175-109抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HL-60, U-937纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu
BAFF (B-cell activating factor), encoded by TNFSF13B and also known as BLyS or CD257, is a 285-aa type-II transmembrane glycoprotein of the TNF ligand superfamily produced by monocytes, dendritic cells, macrophages and activated T cells that can be cleaved to a soluble homotrimeric cytokine which, via engagement of three receptors—BAFF-R, TACI and BCMA—delivers survival and maturation signals to B cells, orchestrates T-cell co-stimulation, and drives class-switching and antibody production through NF-κB and PI3K/AKT pathways, while excess BAFF triggers B-cell hyperplasia and autoantibody formation, linking its overexpression to systemic lupus erythematosus, Sjögren syndrome, rheumatoid arthritis and multiple myeloma, and making BAFF antagonism (e.g., belimumab) a validated therapeutic strategy.
免疫印迹
WB result of BAFF Recombinant Rabbit mAb
Primary antibody: BAFF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: HL-60 whole cell lysate 20 µg
Lane 3: U-937 whole cell lysate 20 µg
Negative control: Daudi whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 30-35 kDa
This blot was developed with high sensitivity substrate
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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