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Rabbit抗原名称
Choline Acetyltransferase/ChAT分子别名
Choline O-acetyltransferase; CHOACTase; Choline acetylase免疫原
Synthetic Peptide细胞定位
Synapse, Cytoplasm, NucleusAccession
P28329克隆号
S-2561-17抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Human brain, mouse brain, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:1000 Hu, Ms, Rt
Choline acetyltransferase (ChAT) is a 69-kDa cytosolic transferase enzyme encoded by the CHAT gene that catalyzes the single-step acetylation of choline with acetyl-CoA to synthesize the neurotransmitter acetylcholine (ACh) in the presynaptic terminals of cholinergic neurons throughout the central and peripheral nervous systems; it is synthesized in the soma, trafficked to nerve endings via axoplasmic flow, exists in soluble and membrane-bound pools, displays alternative splicing that can generate larger nuclear variants, and is considered the most specific phenotypic marker for cholinergic neurons whose functional integrity underlies memory, cognition, arousal, and motor control, while its loss or dysfunction is closely linked to the cholinergic deficits seen in Alzheimer’s disease, amyotrophic lateral sclerosis, schizophrenia, and other neurodegenerative or neuropsychiatric disorders .
免疫印迹
WB result of Choline Acetyltransferase/ChAT Recombinant Rabbit mAb
Primary antibody: Choline Acetyltransferase/ChAT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Negative control: mouse kidney lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 70 kDaWB result of Choline Acetyltransferase/ChAT Recombinant Rabbit mAb
Primary antibody: Choline Acetyltransferase/ChAT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Choline Acetyltransferase/ChAT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Choline Acetyltransferase/ChAT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Choline Acetyltransferase/ChAT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Choline Acetyltransferase/ChAT in tumor tissue.
Expression of Choline Acetyltransferase/ChAT in human tissue.
Expression of Choline Acetyltransferase/ChAT in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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