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宿主来源
Rabbit抗原名称
IL-18分子别名
Interleukin-18; Iboctadekin; Interferon gamma-inducing factor (IFN-gamma-inducing factor); Interleukin-1 gamma (IL-1 gamma); IGIF; IL1F4; IL18免疫原
Recombinant Protein细胞定位
Secreted, CytoplasmAccession
Q14116克隆号
S-2142-2抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
PC-3, A431, HeLa, DU 145纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:2000 Hu
Interleukin-18 (IL-18) is a 18 kDa pro-inflammatory cytokine of the IL-1 superfamily, constitutively expressed by macrophages, dendritic cells, and epithelial cells as an inactive 24 kDa pro-peptide that is cleaved by caspase-1 within the inflammasome to its mature form; once secreted, IL-18 binds the heterodimeric receptor IL-18Rα/IL-18Rβ and recruits the adaptor MyD88 to activate NF-κB and MAPK pathways, synergizing with IL-12 to drive IFN-γ production from Th1 cells and NK cells and promote cell-mediated immunity, but when unchecked it contributes to the pathogenesis of autoimmune diseases such as rheumatoid arthritis, inflammatory bowel disease, and type 1 diabetes, making it both a biomarker and therapeutic target.
免疫印迹
WB result of IL-18 Recombinant Rabbit mAb
Primary antibody: IL-18 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: DU 145 whole cell lysate 20 µg
Negative control: Raji whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 20 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti- IL-18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti- IL-18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti- IL-18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti- IL-18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- IL-18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti- IL-18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of IL-18 in tumor tissue.
Expression of IL-18 in human tissue.
Expression of IL-18 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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