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Rabbit抗原名称
iNOS分子别名
Nitric oxide synthase, inducible; Hepatocyte NOS (HEP-NOS); Inducible NO synthase (Inducible NOS; iNOS); NOS type II; Peptidyl-cysteine S-nitrosylase NOS2; NOS2A; NOS2免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P35228克隆号
S-2493-61抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:250 Hu, Ms, Rt ICC 1:100 Ms
Inducible nitric oxide synthase (iNOS) is a ~131 kDa homodimeric flavo-hemoprotein that, upon transcriptional up-regulation by cytokines such as IFN-γ, TNF-α and LPS via NF-κB and STAT pathways, catalyzes the NADPH- and O₂-dependent five-electron oxidation of L-arginine to generate large, sustained fluxes of nitric oxide (NO) and citrulline; this Ca²⁺-independent, calmodulin-bound enzyme localizes in cytosol and peroxisomes of macrophages, hepatocytes, chondrocytes and many other cell types, where the produced NO acts as a microbicidal, tumoricidal and immunomodulatory radical that can react with superoxide to form peroxynitrite, leading to protein tyrosine nitration, lipid peroxidation and DNA damage, thereby contributing to antimicrobial defense, vasodilatory shock in sepsis, inflammatory arthritis, neurotoxicity and carcinogenesis, while its expression is tightly controlled by transcriptional, post-transcriptional (mRNA stability, microRNAs) and post-translational (ubiquitin-proteasomal degradation, phosphorylation) mechanisms to balance beneficial and cytotoxic NO levels.
免疫印迹
WB result of iNOS Recombinant Rabbit mAb
Primary antibody: iNOS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Caco-2 whole cell lysate 40 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 131 kDa
Observed MW: 130 kDa
This blot was developed with high sensitivity substrateWB result of iNOS Recombinant Rabbit mAb
Primary antibody: iNOS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated RAW264.7 whole cell lysate 20 µg
Lane 2: RAW264.7 treated with 1 µg LPS for 6 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 131 kDa
Observed MW: 130 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-iNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-iNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-iNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-iNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-iNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC analysis of Raw264.7 cells treated with LPS (1 μg/mL, 6h) (top panel) and untreated Raw264.7 cells (below panel). Anti-iNOS antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of iNOS in tumor tissue.
Expression of iNOS in human tissue.
Expression of iNOS in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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