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Insulin Receptor β Recombinant Rabbit mAb (S-2605-10)

Insulin receptor,IR,CD220,INSR

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6272
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Insulin Receptor β

  • 分子别名

    Insulin receptor; IR; CD220; INSR

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Lysosome, Endosome, Cell membrane

  • Accession

    P06213

  • 克隆号

    S-2605-10

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms

  • 阳性样本

    HepG2, HEK-293, HeLa, HCT 116, MCF7, NIH/3T3

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:500-1:1000 Hu, Ms
背景介绍

  • The insulin receptor β-subunit is the 95 kDa transmembrane tyrosine kinase moiety of the heterotetrameric insulin receptor, spanning the plasma membrane once with its short extracellular domain stabilizing disulfide linkage to the α-subunit and its large cytoplasmic tail harboring an ATP-binding site and catalytic domain that, upon insulin-induced autophosphorylation of specific tyrosines such as Y1158, Y1162 and Y1163, recruits and phosphorylates IRS and Shc adaptor proteins to initiate PI3K-Akt and MAPK signaling cascades governing glucose uptake, glycogen synthesis, lipogenesis and cell growth, while mutations in the β-subunit that impair kinase activity or endocytosis are linked to severe insulin resistance syndromes including Rabson–Mendenhall and type A diabetes.

  • 免疫印迹

    • WB result of Insulin Receptor β Recombinant Rabbit mAb
      Primary antibody: Insulin Receptor β Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HepG2 whole cell lysate 20 µg
      Lane 2: HEK-293 whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Lane 4: HCT 116 whole cell lysate 20 µg
      Lane 5: MCF7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 156 kDa
      Observed MW: 105 kDa
      This blot was developed with high sensitivity substrate

    • WB result of Insulin Receptor β Recombinant Rabbit mAb
      Primary antibody: Insulin Receptor β Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 156 kDa
      Observed MW: 105 kDa
      This blot was developed with high sensitivity substrate

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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