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Rabbit抗原名称
KL分子别名
Klotho免疫原
Synthetic Peptide细胞定位
Secreted, Cell membraneAccession
Q9UEF7克隆号
S-2245-169抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Jurkat, K562, mouse kidney, mouse testis, rat kidney, rat testis纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt
KL protein, also known as α-Klotho, is a type-I single-pass transmembrane protein encoded by the KL gene on chromosome 13, featuring a short intracellular tail and a large extracellular region composed of tandem KL1 and KL2 glycosidase-like domains that can be proteolytically cleaved by ADAM10 and other proteases to release a soluble, circulating hormone-like form. Initially discovered as an aging-suppressor, KL functions as a coreceptor for FGF23 to regulate phosphate, calcium, and vitamin D metabolism, while its soluble form modulates insulin/IGF-1 and Wnt signaling, suppresses oxidative stress and inflammation, and enhances synaptic plasticity and cognition; consequently, KL overexpression extends lifespan and improves healthspan in mice, whereas its deficiency accelerates aging phenotypes such as arteriosclerosis, osteoporosis, sarcopenia, and cognitive decline, and human KL variants are associated with hypertension, chronic kidney disease, and age-related disorders .
免疫印迹
WB result of KL Recombinant Rabbit mAb
Primary antibody: KL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 116 kDa
Observed MW: 58 kDaWB result of KL Recombinant Rabbit mAb
Primary antibody: KL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 116 kDa
Observed MW: 55, 130 kDaWB result of KL Recombinant Rabbit mAb
Primary antibody: KL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 116 kDa
Observed MW: 55-130 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-KL antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-KL antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-KL antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-KL antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-KL antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-KL antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-KL antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-KL antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of KL in tumor tissue.
Expression of KL in human tissue.
Expression of KL in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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