M-Cadherin/Cadherin 15 Recombinant Rabbit mAb (S-2261-74)

Cadherin-15,Cadherin-14,Muscle cadherin (M-cadherin),Cdh14,Cdh15

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6270

规格

25μl100μl1ml

数量

产品介绍

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产品规格

应用	稀释度	推荐种属
WB	1:1000	Ms
ICC	1:500	Ms

背景介绍

M-Cadherin (Cadherin-15), a classical calcium-dependent cell-adhesion glycoprotein, is primarily expressed in developing skeletal muscle, satellite cells and cerebellum, where it mediates homophilic binding to organize myoblast fusion, muscle fiber formation and neuronal layer integrity by assembling adherens junctions through catenin-dependent linkage to the actin cytoskeleton ; despite its name, germline deletion in mice is largely compensated by N-cadherin, yet human mutations causing loss of adhesive function are linked to autosomal-dominant mental retardation-3, underscoring a non-redundant role in synaptic and cognitive development .

免疫印迹
- WB result of M-Cadherin/Cadherin 15 Recombinant Rabbit mAb
  Primary antibody: M-Cadherin/Cadherin 15 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: C2C12 whole cell lysate 20 µg
  Negative control: NIH/3T3 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 86 kDa
  Observed MW: 85, 110, 135 kDa
  This blot was developed with high sensitivity substrate
免疫细胞化学
- ICC shows positive staining in C2C12 cells (top panel) and negative staining in NIH/3T3 cells (below panel). Anti- M-cadherin/cadherin-15 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).