MFF Recombinant Rabbit mAb (S-2330-28)

Mitochondrial fission factor,C2orf33

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6269

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

MFF
分子别名

Mitochondrial fission factor; C2orf33
免疫原

Synthetic Peptide
细胞定位

Mitochondrion
Accession

Q9GZY8
克隆号

S-2330-28
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

MCF7, DU 145, A549, HeLa, T-47D, mouse brain, rat brain
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu

背景介绍

MFF (Mitochondrial Fission Factor) is an evolutionarily conserved, tail-anchored outer-mitochondrial-membrane receptor that serves as the principal platform for recruiting the cytosolic GTPase Drp1 to sites of future organelle division, thereby initiating the oligomerization of Drp1 into a helical collar that constricts and finally severs the mitochondrion in a GTP-driven reaction, while also integrating peroxisomal fission, controlling mitochondrial size, number and distribution, and acting as a crucial node for post-translational and lipid-mediated signals that tune the balance between fission and fusion in response to metabolic stress, cell-cycle cues and pathological stimuli such as those observed in cancer and cardiovascular disease.

免疫印迹
- WB result of MFF Recombinant Rabbit mAb
  Primary antibody: MFF Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: MCF7 whole cell lysate 20 µg
  Lane 2: DU 145 whole cell lysate 20 µg
  Lane 3: A549 whole cell lysate 20 µg
  Lane 4: HeLa whole cell lysate 20 µg
  Lane 5: T-47D whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 38 kDa
  Observed MW: 25-45 kDa
- WB result of MFF Recombinant Rabbit mAb
  Primary antibody: MFF Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 38 kDa
  Observed MW: 35 kDa
  This blot was developed with high sensitivity substrate
- WB result of MFF Recombinant Rabbit mAb
  Primary antibody: MFF Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 38 kDa
  Observed MW: 35, 48 kDa
  This blot was developed with high sensitivity substrate
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-MFF antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).